ISSN: 2157-7064
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Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32608, USA
Dr. Richard C. Hill is a board-certified veterinary nutritionist and associate professor at the University of Florida's College of Veterinary Medicine. Since 1993, he has been teaching small animal internal medicine and nutrition to veterinary students. Before his academic career, Dr. Hill worked in small animal private practice in the United Kingdom for five years. He completed a residency at the University of Pennsylvania and earned his Ph.D. at the University of Florida. TODAY'S VETERINARY PRACTICE Dr. Hill's research focuses on the nutrient and energy requirements and physiology of working and pet dogs, cats, and endangered marine mammals. He is a coauthor of the current National Research Council nutrient recommendations for feeding dogs and cats. TODAY'S VETERINARY PRACTICE Some of his notable publications include: "Proximate nutrient analyses of four species of submerged aquatic vegetation consumed by Florida manatee (Trichechus manatus latirostris) compared to romaine lettuce (Lactuca sativa var. longifolia)," published in the Journal of Zoo and Wildlife Medicine. UF VET MED "Comparison of the guaranteed analysis with the measured nutrient composition of commercial pet foods," published in the Journal of the American Veterinary Medical Association. UF VET MED For more information on Dr. Hill's work and publications, you can visit his profile on the University of Florida's College of Veterinary Medicine website. UF VET MED
Research Article
Modification of a Targeted Metabolomics Method Using Hydrophilic Interaction Liquid Chromatography-High Resolution Mass Spectrometry to Measure Nine Purines in Commercial Dog Foods
Author(s): Chie Saito Ishii*, Karen C Scott, Carolina Abrahan, Joy G. Cagmat, Taylor A. Harmon, Yann Queau, Vincent Biourge, Timothy J. Garrett and Richard C Hill
Urate is the third most common component of canine uroliths. Dogs with a genetic mutation or hepatic dysfunction are prone to forming urate uroliths because they excrete more poorly soluble urate as the end product of purine metabolism. Dietary purine restriction has been reported to reduce urinary urate concentrations in dogs, but the amount and types of purine in commercial diets have not been well documented. A previous targeted metabolomics method was modified to measure eleven purine metabolites in commercial dog foods. The method utilized hydrophilic interaction liquid chromatography-high-resolution tandem mass spectrometry with stable isotope-labeled internal standards. The within-day and between-day coefficient of variation of each purine metabolite concentration in quality control dog food samples averaged 6% (range: 4%-13%) and 15% (range: 11%-19%), respectively. Recoveries .. View More»
DOI:
10.35248/2157-7064.23.14.539