Journal of Molecular Imaging & Dynamics
Open Access

Department of Microbiology and Immunology, University of Western Ontario, London, Canada

Publications

• Editorial   
  AFM Meets Magnetic Tweezers to Probe Protein Dynamics under A Wide Range of Forces
  Author(s): Richter Nadia*
  
  Magnetic Tweezers (MT) is a single-molecule technology that allows researchers to investigate the mechanical characteristics of nucleic acids and protein-nucleic acid interactions in real time. A MT system, contrary to its name, does not manage items in the same way that a pair of macroscopic tweezers does. Because it uses the magnetic field gradient generated by permanent magnets to pull micrometer-size magnetic beads, the position of which can be tracked in three dimensions using pictures given by an inverted optical microscope and CMOS or CCD cameras, an MT is truly a magnetic puller. Fixed beads are used to determine the height of a glass surface as a zero-height reference (Refbead). With a DNA molecule with proper functionalization at their ends, DNA beads are anchored to the glass surface. The.. View More»
  

  Abstract HTML PDF