Journal of Clinical and Cellular Immunology
Open Access

Department of General Surgery, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China

Publications

• Research Article   
  Method for Constructing Human iPS Cell Line RZPBi001 from Peripheral Blood Cells
  Author(s): Xiaoqing Chen, Yuling Lua, Rong Ying, Weiming Yua, Pengfei Tian, Dongming Hua and Yu Feng*
  
  Patient-derived hiPSCs could generate indefinitely illness-related cells and organoids, allowing research scientist to reproduce some pathologic change of human sickness models in petri dishes. Whereas, dermal fibroblasts require some traumatic injury to obtain and have the risky of accumulating variations due to unveil to external stimulus such as UV light. Meanwhile, peripheral blood is regarded to be a more excellent source of somatopiasm because it is less damage to collect and its mutation load is significantly lower than that of skin tissue. In this paper, we describe the successful construction of non-integration hiPSCs form human peripheral blood cells by Sendai virus. A healthy adult female of Han nationality from China contributed her Peripheral Blood Mononuclear Cells (PBMCs). Non-integrating Sendai virus recombinant PBMCs with human OSKM (Oct4, Sox2.. View More»
  DOI: 10.35248/2155-9899.23.14.686

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