Journal of Probiotics & Health
Open Access

School of Nutrition and Food Sciences, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA

Biography

Ryan Page is  Working in University of Maribor located in slovenia. Sabina Fijan author of peer reviewed journals and area of expertise, as a Researcher credits with many publications in national and international journals

Publications

• Research Article   
  Cell Wall Integrity and Protoplast Formation of the Probiotic Lactobacillus acidophilus through Fluorescent Staining and Fluorescence Microscopy
  Author(s): Ryan Page, David Burk and Kayanush Aryana*
  
  The identification of protoplast of bacterial cells has previously utilized phase contrast microscopy. This method determines protoplast by their size and change in shape. A more verifiable method can be used utilizing fluorescent stains that target the specific cellular components. The goal of this study was to utilize fluorescence microscopy techniques to determine the presence or absence of bacterial cell walls in the probiotic Lactobacillus acidophilus, after exposure to cell wall digestive enzymes. Bacterial cells were treated with different concentrations of lysozyme [0, 175, 250, 425 μg/ml] and were incubated at 37°C for ten minutes. Following lysozyme treatment cells were fluorescently stained with different concentrations (1x, 2x, 10x, and 100x) of two fluorescent dyes, Wheat-germ agglutinin (WGA) and Hoechst 33342. The WGA [CF®.. View More»
  

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