Journal of Cell Science & Therapy
Open Access

Department of Otolaryngology-Head and Neck Surgery, Indiana University School of Medicine (IUSM), Indianapolis, IN, USA

Publications

• Research Article   
  Impact of Needle Selection on Survival of Muscle-Derived Cells When Used for Laryngeal Injections
  Author(s): Oluwaseyi Awonusi, Zachary J. Harbin, Sarah Brookes, Lujuan Zhang, Samuel Kaefer, Rachel A. Morrison, Sharlé Newman, Sherry Voytik-Harbin and Stacey Halum*
  
  Objective: To describe how differing injector needles and delivery vehicles impact Autologous Muscle-Derived Cell (AMDC) viability when used for laryngeal injection. Methods: In this study, adult porcine muscle tissue was harvested and used to create AMDC populations. While controlling cell concentration (1 × 107 cells/ml), AMDCs including Muscle Progenitor Cells (MPCs) or Motor Endplate Expressing Cells (MEEs) were suspended in either phosphate-buffered saline or polymerizable (in-situ scaffold forming) type I oligomeric collagen solution. Cell suspensions were then injected through 23- and 27-gauge needles of different lengths at the same rate (2 ml/min) using a syringe pump. Cell viability was measured immediately after injection and 24- and 48-hours post-injection, and then compared to baseline cell viabi.. View More»
  DOI: 10.35248/2157-7013.22.14.377

  Abstract HTML PDF