Journal of Proteomics & Bioinformatics
Open Access

Department of Bioinformatics & Biostatistics, University of Louisville, Louisville, Kentucky, USA

Publications

• Rapid Communication   
  Interactive Web Tool for Standardizing Proteomics Workflow for Liquid Chromatography-Mass Spectrometry Data
  Author(s): Sudhir Srivastava, Michael Merchant, Anil Rai and Shesh N. Rai*
  
  Introduction: The proteomics experiments involve several steps and there are many choices available for each step in the workflow. Therefore, standardization of proteomics workflow is an essential task for design of proteomics experiments. However, there are challenges associated with the quantitative measurements based on liquid chromatography-mass spectrometry such as heterogeneity due to technical variability and missing values. Methods: We introduce a web application, Proteomics Workflow Standardization Tool (PWST) to standardize the proteomics workflow. The tool will be helpful in deciding the most suitable choice for each step of the experimentation. This is based on identifying steps/choices with least variability such as comparing Coefficient of Variation (CV). We demonstrate the tool on data with categorical and continuous variables.. View More»
  DOI: 10.35248/0974-276X.19.12.500

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