Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Department of Applied Biology, Ministry of Agriculture, Addis Ababa, Ethiopia
Review
Review on the Role of Gene Editing Technology for the Targeted Animal Diseases
Author(s): Ermias Menbere*, Ketema Tafess, Abdi Ahmed, Wubishet Zewude and Sisay Getachew
Gene editing is a technique of improving the genetic makeup of organisms by deletion of infected alleles, the wild type of sequence modified or integration of exogenous Deoxyribonucleic Acid (DNA) to obtain new gene function. Gene editing is achieved by zinc finger nucleases, transcriptional activating like effector nucleases and recently with newly modified genetic tools known as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9) is used to edit the non-functional genes into functional genes. The natural defense mechanisms of prokaryotes against phage invasion coordinated in three stages, adaptation, CRISPR Ribonucleic Acid (crRNA) synthesis and targeted interference. The mechanisms of CRISPR/Cas9 actions completed three parts that are recognition, cleavage and repairing process. The engineered Guide RNA (gRNA) components derived from the natural crRNA, the guidi.. View More»