Journal of Chromatography & Separation Techniques
Open Access

Department of Chemistry, GITAM School of Science, GITAM (Deemed to be University), Rudraram, Hyderabad, Telangana, India

Biography

Siva Jyothi N is urrenly working in Department of Chemistry, GITAM School of Science for the GITAM (Deemed to be University) located at  Rudraram, Hyderabad, Telangana, India,

Publications

• Short Communication   
  Development of Validation and Stability Indicating Method of Anti-HIV Dolutegravir Drug and its Related Impurities by Using RP-HPLC
  Author(s): Venkatnarayana M* and Siva Jyothi N
  
  A simple, rapid and robust reverse phase HPLC method was developed and validated for the determination of impurities in Dolutegravir drug substance. The main aim of this study is to reduce the time consumption and to develop and validate a less expensive method by using HPLC. The chromatographic separation of Dolutegravir and its related impurities is carried out by using C8 column (150 × 4.6 mm), 5µm with 0.1% trifluoroacetic acid in water as mobile phase A, methanol as mobile phase B. The flow rate is 1.0 mL/min with gradient elution mode and the wave length for detection is 240 nm (UV detector). The developed method was validated and proved that the method was specific, accurate and precise as per ICH. The system suitability criteria found to be within the limits. The limit of detection and limit of quantification demonstrate that the method is sensitive. The linearity .. View More»
  DOI: 10.35248/2157-7064.20.11.426

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