ISSN: 0974-276X
Department of Biological Studies, Flinders University, Adelaide, Australia
Short Communication
Role of Top-Down Proteomics for Mass Measurement using Ion Trapping Mass Spectrometer
Author(s): Susan Williams*
Top-down proteomics is a method of protein identification that uses an ion trapping mass spectrometer to store an
isolated protein ion for mass measurement and tandem mass spectrometry analysis, or other protein purification
methods such two-dimensional gel electrophoresis with MS/MS. Through the study of intact proteins, top-down
proteomics may detect and quantify distinct proteoforms. The name comes from the fact that DNA sequencing is
done in a similar way. Intact proteins are electrospray ionized and trapped in a Fourier transform ion cyclotron
resonance (Penning trap), quadrupole ion trap (Paul trap), or Orbit rap mass spectrometer during mass spectrometry.
Electron-capture dissociation or electron-transfer dissociation are used to fragment samples for tandem mass
spectrometry. Prior to mass spectrometry-based proteomics, effective fractionation is necessary .. View More»
DOI:
10.35248/0974-276X.22.15.572