Journal of Clinical Chemistry and Laboratory Medicine
Open Access
ISSN: 2736-6588
ISSN: 2736-6588
Department of Veterinary Science, Nanning City Aquatic Animal Husbandry and Veterinary Station, Shajing Subdistrict Office, Jiangnan District, Guangxi, China
Research Article
Screening Candidate Genes Related to Psoas Muscle Traits in Debao and Landrace Pigs Based on Transcriptome Analysis
Author(s): Chang-Yi Chen, Su-xian Zeng, Yuan-Ding Ma, Jun-Wen Zheng, Xin Li, Chen-Yong Xiong, Hong-Jin Zhou, Chun-Tao Wei and Zong-Qiang Li*
To identify the important genes that affect the phenotypic differences between the Landrace and Debao pigs, especially the differences in metabolism and muscle growth. Differentially expressed genes of psoas major were detected by mRNA transcriptome sequencing in Landrace and Debao pigs. By extracting the total RNA of the psoas major muscle of the Landrace pig and the Debao pig, purifying the mRNA, constructing the cDNA library, conducting transcriptome sequencing, and then through the sequencing quality evaluation, we know that the sequencing quality of this study is relatively high. A total of 17,943 genes were detected in all samples, including 17,870 known genes and 73 new genes. Defined genes with |log2FC| greater than 2 and Q-value less than 0.001, and screened them as significantly differentially expressed genes. A total of 1661 differentially expressed genes were screened from.. View More»
DOI:
10.35248/ JCCLM.22.05.218