Journal of Alcoholism & Drug Dependence
Open Access
ISSN: 2329-6488
ISSN: 2329-6488
Department of Legal Medicine, Kanagawa Dental University, Yokosuka, Kanagawa 238-8580, Japan
Takeshi Haseba is affiliated to Department of Legal Medicine, Kanagawa Dental University, Yokosuka, Kanagawa 238-8580, Japan. He is a recipient of many awards and grants for his valuable contributions and discoveries in major area of subject research. His international experience includes various programs, contributions and participation in different countries for diverse fields of study. His research interests reflect in his wide range of publications in various national and international journals.
Research Article
Role of Class III Alcohol Dehydrogenase (ADH3) in Renal Pathological Changes Induced by Chronic Alcohol Consumption in Mice
Author(s): Midori Katsuyama*, Takahisa Okuda, Yoshihiro Sasaki, Masamichi Ishizaki, Kentaro Wada, Motoyo Maruyama, Toshio Akimoto, Youkichi Ohno and Takeshi Haseba
Aims: Our study investigated ADH3 role in renal pathological changes induced by chronic alcohol consumption (CAC).
Methods: Nine-week-old malewild-type (WT) and ADH3-deficient (Adh3−/−) mice were administered 10% ethanol solution for 1 month. Renal histological and ultrastructural analyses were performed by light microscopy on periodic acid–Schiff (PAS) staining and electron microscopy. The tissue ADH3 localization was examined by immunohistochemistry using a specific antibody against mouse ADH3. Urine and serum biochemical analyses were performed.
Results: The PAS-stained microvilli in the proximal tubules were significantly low in the WT mice after CAC (WT (E)) as compared to the Adh3−/− mice (Adh3−/− (E)). The elect.. View More»
DOI:
10.35248/2329-6488.21.9.340