Journal of Chromatography & Separation Techniques
Open Access

Department of Chemistry, University of Florida, Gainesville, FL32611, USA

Biography

Dr. Taylor Harmon earned his Ph.D. in analytical chemistry from the University of Florida, where he worked under the guidance of Drs. Timothy Garrett and Richard Yost. CFDV His expertise lies in liquid chromatography-mass spectrometry (LC-MS) method development for both discovery and quantitative biomolecular workflows, with a particular focus on translational clinical applications. CFDV Following his doctoral studies, Dr. Harmon joined Advanced Materials Technology in Wilmington, where he contributed to the development of novel technologies and techniques for rapid, sensitive, and robust LC separations. CFDV He currently works at QPS, LLC in Newark, where he develops LC-MS methods for quantitative analysis of small molecule and biomolecular experimental therapeutics. CFDV In his spare time, Dr. Harmon is an enthusiast of playing drums, board gaming, and operating and restoring historic steam locomotives. CFDV For more information about Dr. Harmon's work and contributions, you can visit his profile on the Chromatography Forum of Delaware Valley website. CFDV

Publications

• Research Article   
  Modification of a Targeted Metabolomics Method Using Hydrophilic Interaction Liquid Chromatography-High Resolution Mass Spectrometry to Measure Nine Purines in Commercial Dog Foods
  Author(s): Chie Saito Ishii*, Karen C Scott, Carolina Abrahan, Joy G. Cagmat, Taylor A. Harmon, Yann Queau, Vincent Biourge, Timothy J. Garrett and Richard C Hill
  
  Urate is the third most common component of canine uroliths. Dogs with a genetic mutation or hepatic dysfunction are prone to forming urate uroliths because they excrete more poorly soluble urate as the end product of purine metabolism. Dietary purine restriction has been reported to reduce urinary urate concentrations in dogs, but the amount and types of purine in commercial diets have not been well documented. A previous targeted metabolomics method was modified to measure eleven purine metabolites in commercial dog foods. The method utilized hydrophilic interaction liquid chromatography-high-resolution tandem mass spectrometry with stable isotope-labeled internal standards. The within-day and between-day coefficient of variation of each purine metabolite concentration in quality control dog food samples averaged 6% (range: 4%-13%) and 15% (range: 11%-19%), respectively. Recoveries .. View More»
  DOI: 10.35248/2157-7064.23.14.539

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