Journal of Medical Diagnostic Methods
Open Access
ISSN: 2168-9784
+44 1300 500008
ISSN: 2168-9784
+44 1300 500008
Department of Microbiology, Immunology, and Tropical Medicine, The George Washington University Medi, United States of America
Research Article
A Point-of-Care Diagnostic Platform for Detecting Host Immune Activation due to Infections: Cytocapture of Biomarkers In Situ (CyBIS)
Author(s): Timothy A. McCaffrey*, Richard Wargowsky, Kevin Jaatinen, Faisal Al Munajjed, Farris Alqalam, John Perkins, Zane Hayden, Mary Pasquale, Grace Holloway, Jennifer Goldman, Zachary Falk, Tisha Jepson, David Yamane, John LaFleur, Andrew Meltzer, Soroush Shahamatdar, Ryan Heidish, Eduard Shaykhinurov, Aditya Loganathan, Tarun Loganathan and Taylor Bolden
The prompt diagnosis of internal infections is an important, but surprisingly difficult, component of healthcare. Existing clinical and laboratory tests, such as complete blood counts, are low accuracy (~70%), time consuming (>90 min), and require expensive blood analyzers. More sensitive and specific tests, such as PCR or sequencing, require access to a sample of the infected tissue, genomic data for all potential pathogens and expensive equipment. Culturing organisms introduces its own biases and is prone to false positives due to contamination. We describe a device for the rapid isolation of neutrophils and measurement of neutrophil elastase activity to provide a measure of host immune activity toward a broad range of infectious agents. CyBIS (Cytocapture of Biomarkers In Situ) is a Point-of-Care (POC) device that isolates neutrophils from a small volume of blood (75 ul) using a.. View More»