ISSN: 0974-276X
Department of Botany, Sri Krishnadevaraya University, Anantapuram-515003, India
Research
In silico Mining of EST-SSRs from the Drought Tolerant ESTs in Safflower
Author(s): Sudhakar C*, Thippeswamy M, Sivakumar M, Sudhakarbabu O and Dudhe MY
Drought stress is a major abiotic factor causing yield loss in safflower and limited studies have been carried out to dissect the molecular mechanism at EST-SSR level. In this study, a possible relationship between simple sequence repeats (SSRs) distribution and drought stress was studied using three EST libraries of safflower, Ct-D-EST, Ct-NEST and Co-EST. The Ct-D-EST was generated from drought tolerant safflower cultivar A-1; Ct-N-EST and Co-EST were the EST datasets of safflower and its wild progenitor C. oxycanthus, respectively. In total, 156 (45%), 1194 (5%) and 1550 (9%) EST-SSRs were mined from Ct-D-EST, Ct-N-EST and Co-EST libraries, respectively. Comparison of EST-SSRs from Ct-D-EST with that of SSRs from other two libraries showed reasonable differences for each class of repeats. Large variations were observed for dinucleotide repeats in all the libraries. In drought EST-S.. View More»
DOI:
10.35248/0974-276X.19.12.506