Journal of Chromatography & Separation Techniques
Open Access

Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL 32610, USA

Biography

Dr. Timothy J. Garrett is an Associate Professor in the Department of Pathology, Immunology, and Laboratory Medicine at the University of Florida's College of Medicine in Gainesville, Florida. He also serves as the Chief of Experimental Pathology and Co-Editor-in-Chief of the Journal of Mass Spectrometry and Advances in the Clinical Lab (JMSACL). GARRETT LAB Dr. Garrett earned his undergraduate degree in Chemistry from the University of Georgia, graduating summa cum laude with highest honors. He completed his Ph.D. at the University of Florida under the mentorship of Dr. Richard A. Yost, where he developed the first imaging mass spectrometer based on an ion trap, focusing on the disposition and characterization of phospholipids in brain tissue. GARRETT LAB His research interests include the application and development of mass spectrometry techniques for clinical research, with a particular emphasis on metabolomics and lipidomics. Dr. Garrett has developed technologies such as LipidMatch, an open-source platform for lipid identification, and Iterative-Exclusion (IE Omics), which enhances lipid annotation. GARRETT LAB Throughout his career, Dr. Garrett has supervised 18 graduate students and three post-doctoral fellows, with 11 Ph.D. graduates from his group. He has served as Principal Investigator or Co-Principal Investigator on grants totaling over $39 million and has authored over 100 peer-reviewed publications. GARRETT LAB For a comprehensive list of Dr. Garrett's publications, please refer to his Google Scholar profile. GOOGLE SCHOLAR

Publications

• Research Article   
  Modification of a Targeted Metabolomics Method Using Hydrophilic Interaction Liquid Chromatography-High Resolution Mass Spectrometry to Measure Nine Purines in Commercial Dog Foods
  Author(s): Chie Saito Ishii*, Karen C Scott, Carolina Abrahan, Joy G. Cagmat, Taylor A. Harmon, Yann Queau, Vincent Biourge, Timothy J. Garrett and Richard C Hill
  
  Urate is the third most common component of canine uroliths. Dogs with a genetic mutation or hepatic dysfunction are prone to forming urate uroliths because they excrete more poorly soluble urate as the end product of purine metabolism. Dietary purine restriction has been reported to reduce urinary urate concentrations in dogs, but the amount and types of purine in commercial diets have not been well documented. A previous targeted metabolomics method was modified to measure eleven purine metabolites in commercial dog foods. The method utilized hydrophilic interaction liquid chromatography-high-resolution tandem mass spectrometry with stable isotope-labeled internal standards. The within-day and between-day coefficient of variation of each purine metabolite concentration in quality control dog food samples averaged 6% (range: 4%-13%) and 15% (range: 11%-19%), respectively. Recoveries .. View More»
  DOI: 10.35248/2157-7064.23.14.539

  Abstract HTML PDF