Andrology-Open Access
Open Access

Department of Histology and Embryology, Yeni Yuzyil University, Faculty of Medicine, Istanbul, Turkey

Publications

• Research Article   
  Sperm Chromatin Condensation Level: Depending on Fixatives
  Author(s): Tulay Irez* and Tugce Durmus
  
  Sperm chromatin undergoes structural changes during spermatogenesis causing high condensation. This nuclear exchange is considered to be a predictor of sperm-fertilization capacity and pregnancy outcome. Various studies have shown a relationship between male infertility and sperm protamine deficiency, and it has been suggested that it may be an important determinant of sperm DNA integrity. Acidic aniline blue staining is often used with glutaraldehyde fixation and stains histones due to lysine’s in the sperm nucleus. Research has shown that the level of histones in sperm nuclei is closely related to DNA damage and infertility. Sperm chromatin condensation is expressed by the presence of protamine in sperm nucleus, that is, the absence of histone. In this study, we investigated how the aniline blue staining method can correctly determine sperm chromatin condensation when differen.. View More»
  DOI: 10.35248/2167-0250.22.11.262

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