Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, New Delhi, India
Research Article
Thiourea Derivatives Restore Dysfunctional Mitochondria in Chronic Hepatitis B Infection
Author(s): Jitendra Kumar*, Purnima Tyagi, Raghwendra Pratap Singh, Akhilesh k Saini, Deepti Sharma, Jaswinder Singh Maras* and Vijay Kumar*
Background: Chronic Hepatitis B (CHB) infection and the Hepatitis B virus X protein (HBx) are major risk factors associated with Hepato Cellular Carcinoma (HCC). In CHB infection, HBx induces mitochondrial dysfunction, exhaustion and impaired function in hepatocytes. Restoring hepatocyte health along with reduction in virus replication could be an ideal treatment for CHB. Thiourea derivatives are well known for their antiviral properties, though their effect on mitochondrial and/or hepatocyte health remains obscure. This study focuses on the repurposing of thiourea derivatives (DSA-00, DSA-02 and DSA-09) for hepatocyte replenishment.
Methods: HepG2.2.15 cells were treated with thiourea derivatives, alongside Entecavir (ETV). HBV DNA, RNAs and antigens, measured by qPCR, Quantitative Reverse Transcriptase Polymerase Chain Reaction (.. View More»
DOI:
10.35248/0974-276X.23.16.652