Journal of Clinical Chemistry and Laboratory Medicine
Open Access
ISSN: 2736-6588
ISSN: 2736-6588
Department of Pathology and Pathophysiology, Kunming Medical University, Kunming, China
Research Article
TNFSF15 Inhibits Progression of Diabetic Retinopathy by Blocking Pyroptosis via Interacting with GSDME
Author(s): Hongkun Zhao, Yating Dai, Yunqin Li, Juanjuan Li* and Hua Li*
Diabetic retinopathy is a common microvascular complication of diabetes and a leading cause of blindness. Pyroptosis
has emerged as a mechanism of cell death involved in diabetic retinopathy pathology. This study explored the role
of Gasdermin E (GSDME)-mediated pyroptosis and its regulation by TNFSF15 in diabetic retinopathy. We found
GSDME was upregulated in the progression of diabetic retinopathy. High glucose promoted GSDME-induced
pyroptosis in retinal endothelial cells and retinal pigment epithelial cells, attributable to activation of caspase-3
which cleaves GSDME to generate the pyroptosis-executing N-terminal fragment. TNFSF15 was identified as a
binding partner and inhibitor of GSDME-mediated pyroptosis. TNFSF15 expression was increased by high glucose
but suppressed by the caspase-3 activator Raptinal. Moreover, TNFSF15 protein inhibited high glucose an.. View More»
DOI:
10.35248/2736-6588.24.7.282