Journal of Clinical Chemistry and Laboratory Medicine
Open Access
ISSN: 2736-6588
ISSN: 2736-6588
Department of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia
Research Article
CLINICAL UTILITY OF IMMATURE RETICULOCYTE FRACTION
Author(s): Tiruneh Adane*, Fikir Asrie and Zegeye Getaneh
Automated flow cytometric analysis has led to a significant advance in reticulocyte counting, providing the immature reticulocyte fraction (IRF). IRF is one of the newer parameters of automated hematology analyzers and is a sensitive measure of erythropoiesis. The manual reticulocyte counts enumerate all RNA stained cells and simply puts together immature and mature reticulocytes. It is also laborious and time-consuming. Flow cytometric reticulocyte analysis is more precise and sensitive than the manual count. Besides, the measured fluorescence intensity allows quantification of reticulocyte maturity. IRF gives a basic idea about the marrow erythropoietic activity and its response to drugs and therapy. Moreover, it is simple, quick, cost- effective, reproducible and reliable tool on the automated hematology analyzer. It is also important for the evaluation of aplastic anemia in cases .. View More»