Journal of Glycobiology
Open Access

A Note on Characterization of Disease-Associated with N-linked Glycoproteins

Heldin Joanne^{* *}Correspondence: Heldin Joanne, Department of Biochemistry, Cairo University, Giza, Egypt, Email:

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Description

N-linked glycoprotein plays an important critical role in organic processes, inclusive of cell-to-cell recognition, growth, differentiation and programmed cell death. Specific N-linked glycoprotein adjustments are related to sickness development and identity of those N-linked glycoproteins has capacity to be used in sickness diagnosis, prognosis and prediction of treatments [1]. It summarizes not unusual place techniques for N-linked glycoprotein characterization and packages of those techniques to identity of glycoprotein adjustments related to sickness states and also additionally evaluate the N-linked glycoproteins altered in sicknesses consisting of breast cancers, lung cancers, and prostate cancers. Although assays for those glycoproteins have capacity medical utility, studies is wanted to translate those glycoproteins to medical biomarkers [2-4].

Lectin-affinity chromatography: Glycoproteins or glycopeptides may be affinity remoted with an immobilized glycan-binding protein consisting of lectin. Certain lectins own affinity for specific oligosaccharide moieties and for that reason numerous lectins bind to one of a kind systems of glycans on glycoconjugates. Various lectins had been utilized in glycoprotein isolation. N-glycans, Lens culinaris agglutinin for core-fucosylated N-glycans, Sambucus nigra for sialylated N-glycans. Although lectins do now no longer own absolute specificity, diffused variations in glycoprotein profiles may be detected. Advantages of this feature technique are reversible binding, a couple of affinity selectors and retrievability of glycans for characterization and quantification. For instance, isotopic glycosidase elution, labeling on lectin-column chromatography and iTRAQ 8-plex isobaric tags had been used to become aware of and quantify Nglycosylation in lung cancer.

Hydrazide chemistry: A technique for selective isolation, identity and quantification of N-linked glycoproteins through hydrazide chemistry. This approach includes the conjugation of glycoproteins or glycopeptides to a strong guide after oxidization of the carbohydrates at the glycoproteins/glycopeptides and unique launch of previously N-linked glycopeptides through peptide-N-glycosidase. This approach has been implemented to the identity of glycoprotein adjustments related to one of kind histological subtypes of ovarian cancers competitive prostate cancers, lung cancers and pores and skin cancers.

Boronic acid: Affinity ligands primarily based totally on boronic acid derivatives had been used to seize carbohydrates, nucleosides, glycolipids, RNA and glycoproteins. The precept of boronate-affinity chromatography is that boronic acids can shape covalent ester bonds with cis-diols below fundamental situations in order that glycopeptides may be separated from different molecules. The boronate ester bond may be reversibly hydrolyzed below impartial or acidic situations. The mentioned synthesis of a boronic acid ligand through advent of a terminal acetylene organization into commercially to be had three-aminophenyl boronic acid. Compared to different boronic acid methods, the brand new boronic acid technique confirmed advanced effectiveness in isolating version glycoproteins (ovalbumin and RNase B) from BSA and RNase A with inside the presence of crude E. coli proteins.

A wide variety of N-linked glycoprotein adjustments had been recognized of affiliation with one of a kind sickness the use of glycoproteomic approaches. Studies have centered on not unusualplace cancers inclusive of lung most cancers, Hepatocellular Carcinoma (HCC), pores and skin cancers, prostate cancers, ovarian cancers, and breast cancers. The most cancers-related glycoproteins had been recognized through one of a kind methodology inclusive of lectin-affinity chromatography, hydrazide chemistry and metabolic labeling. Interestingly, odd expressions of sure glycoproteins are related to multiple kinds of most of the cancers [5]. For example, multiplied alpha-1-antichymotrypsin is related to each nonsmall cell lung carcinoma and (Hepatocellular carcinoma) HCC. Upregulated galectin-three-binding protein (Gal3BP or Mac-2 BP) is related to each HCC and ovarian cancers, while downregulated expression of insulin aspect binding protein three is related to each HCC and nonsmall cell lung carcinoma [6].

References

1. Kay Li Q, Gabrielson E, Zhang H. Application of glycoproteomics for the discovery of biomarkers in lung cancer. Proteomics Clin Appl. 2012;6:244-256. 

   [Crossref] [PubMed] [Google Scholar]

2.  Peracaula R, Barrabes S, Sarrats A, Rudd PM, de Llorens R. Altered glycosylation in tumours focused to cancer diagnosis. Dis Markers. 2008;25:207-218. 

   [Crossref]] [PubMed] [Google Scholar]

3. Cummings RD, Kornfeld S. Fractionation of asparagine-linked oligosaccharides by serial lectin-Agarose affinity chromatography. A rapid, sensitive, and specific technique. J Biol. Chem. 1982;257:11235-11240. 

   [PubMed] [Google Scholar]

4.  Wu AM, Song SC, Tsai MS, Herp A. A guide to the carbohydrate specificities of applied lectins-2. Adv Exp Med Biol. 2001;491:551-585.

   [Crossref] [PubMed] [Google Scholar]

5. Catalona WJ, Smith DS, Ratliff TL, Dodds KM. Measurement of prostate-specific antigen in serum as a screening test for prostate cancer. New Engl J Med. 1991;324:1156-1161.

   [Crossref] [PubMed] [Google Scholar]

6. Zhou L, Liu J, Luo F. Serum tumor markers for detection of hepatocellular carcinoma. World J Gastroentero. 2006;12:1175-1181. 

   [Crossref] [PubMed] [Google Scholar]