Entomology, Ornithology & Herpetology: Current Research

Entomology, Ornithology & Herpetology: Current Research
Open Access

ISSN: 2161-0983

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Research Article - (2015) Volume 4, Issue 2

Bio Efficacy of Atalantia monophylla (L) Correa (Rutaceae) against Spodoptera litura Fabricius (Lepidoptera: Noctuidae)

Kathirvelu Baskar1,2*, Chellaih Muthu1 and Savarimuthu Ignacimuthu1
1Entomology Research Institute, Loyola College, Chennai, 600 034, India
2Bioscience Research Foundation, Porur, Chennai, 600 116, India
*Corresponding Author: Kathirvelu Baskar, Entomology Research Institute, Loyola College, Chennai, 600 034, India, Tel: +91-8110009641, Fax: 091-44-2817 5566 Email:

Abstract

Hexane, chloroform and ethyl acetate extracts of Atalantia monophylla were evaluated for their larvicidal and pupicidal activities against Spodoptera litura. Bioefficacy of A. monophylla leaf extracts were studied leaf disc no choice method at 0.5, 1.0, 2.5 and 5.0% concentration of crude extracts and 125, 250, 500 and 1000 ppm concentration of fractions against S. litura. The maximum larvicidal and pupicidal activities were noticed in hexane extract of A. monophylla. The hexane extract exhibited the least LC50 value of 2.01% for larvicidal activity and regression value (R 0.98 and Coefficients 9.33 ± 17.1) for concentration dependent larvicidal activity. The hexane extract was fractionated using increasing polarity of solvent system. Twelve fractions were isolated and were evaluated at four different concentrations. Maximum antifeedant, larvicidal and pupicidal activities was noticed in fraction 9 at 1000 ppm concentration. Ninth fraction exhibited concentration dependent antifeedant and larvicidal activities with liner regression of R 0.93, and 0.93 respectively. Least LC50 value of 340.27 ppm was observed in fraction nine for larvicidal activity. All the concentrations of 9th fraction showed good activity.

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Keywords: Antifeedant; Larvicidal; Pupicidal; Solvent extracts; Fractions

Introduction

Many synthetic pesticides are used to protect the crops from pests attack around the world which lead to environmental damage and human health due residue in fruits and food [1]. Due to this reason, many research studies are being conducted to find an alternative control method for pest management. Plants produced diverse of structurally related compounds. Erythrina alkaloids from the seeds, seed pods and flowers of Erythrina latissima E. Meyer (Fabaceae) exhibited antifeedant activity against Spodoptera littoralis [2]. Clerodendrum inerme L. (Lamiaceae) and Lantana camara L. (Verbenaceae) extracts exhibited many activities including feeding deterrent, mortality, reduction of nymphs and adult longevity, adult emergence and fecundity against Helopeltis theivora [3]. Most of the plant compounds act as digestive enzymes inhibitor (α-amylase, protease, α- and β-glucosidases and lipase) [4]. Baskar et al. [5] stated that plant compound inhibit protein, esterase and glutathione S-transferase enzymes activities of Helicoverpa armigera and Earias vittella.

Cistus ladanifer L. (Cistaceae), Peganum harmala L. (Zygophyllaceae), Ajuga iva L. and Rosmarinus officinalis L. (Lamiaceae) extracts reduced the larval weight, pupation and adult emergence and decreased the protein, carbohydrate, lipid of Plodia interpunctella [6]. Baskar and Ignacimuthu [7] stated that ononitol monohydrate derived from Cassia tora (L.) Roxb. (Fabaceae) exhibited antifeedant, larvicidal and growth inhibitory activities (increased larval-pupal duration and pupicidal activity) against H. armigera and S. litura. Natural compound from Pogostemon cablin (Blanco) Benth (Lamiaceae) exhibited strong antifeedant and insecticidal activities against S. litura and S. exigua [8].

Atalantia monophylla (L.) Corr. is a medicinal plant; oil from the seeds of this plant act as anti-arthritis [9] and also used for chronic rheumatism and paralysis [10]. Boiled leaves are used to cure rheumatoid pain and glandular swelling [11] and decoction from the leaves are used to cure itching and skin problems [12]. Roots are used for antispasmodic [13]. Many insecticidal properties like antifeedant, larvicidal, growth inhibitory, reduced the adult emergence against H. armigera and E. vittella [14,15] were reported.

S. litura is a major destructive pest in most cultivated crops in tropical and subtropical regions. It affects more than 90 families of cruciferous vegetables [16]. Two years host plant survey conducted by Ahmad et al. [17] on S. litura revealed that there were 27 plant species from 25 genera of 14 families are reported. Among them, Gossypium hirsutum L., (Malvaceae), Ricinus communis L., (Euphorbiaceae), Brassica oleracea var. Botrytis L., (Brassicaceae), Colocasia esculenta L., (Araceae), Trianthema portulacastrum L. (Aizoaceae) and Sesbania sesban (Jacq.) W. Wight (Fabaceae) were major host plants for S. litura. Due to its high mobility and reproduction, it affects wide variety of host plants [18]. So we called S. litura as cosmopolitan pest which means unable to manage these pests. Hence, the present study was undertaken to evaluate the antifeedant, larvicidal and pupicidal activities of A. monophylla against S. litura.

Materials and Methods

Plant material

Leaves of A. monophylla were collected from the forest areas of Kancheepuram district of Tamil Nadu, India. The plant was authenticated by a plant taxonomist from the Department of Plant Biology and Biotechnology, Loyola College, Chennai. A voucher specimen [ERIH- 1309] was deposited at the herbarium of Entomology Research Institute, Loyola College,Chennai.

Crude extraction and fraction isolation

Leaves were collected and shade dried at room temperature and ground in a manual mill. The powder was sequentially extracted with hexane, chloroform and ethyl acetate. The powder was soaked in the respective solvents for a period of 48 h with intermittent shaken. The extract was filtered through a Buchner funnel with Whatman number 1 filter paper. The filtrate was evaporated to dryness under reduced pressure using rotary evaporator. The crude hexane extract (50 g) was subjected to column chromatography over silica gel (500 g-acme’s 100–200 mesh) and eluted with hexane followed by the combination of hexane: ethyl acetate and ethyl acetate: acetone ranging from 95:5 to 0:100 and 50:50 to 0:100, respectively. A total of 189 fractions were collected in 200 mL conical flasks and were pooled into twelve fractions using thin layer chromatography (TLC)[14].

Insect culture

Egg masses of S. litura were collected from a groundnut field at Monnavedu village in Tiruvallur District of Tamil Nadu. The egg masses were surface sterilized with 0.02% sodium hypochlorite solution, dried and allowed to hatch. After hatching, the neonate larvae were reared on castor leaves (Ricinus communis) until prepupal stage. Sterilized soil was provided for pupation at room temperature (27+2°C) with 14–10 light: dark photoperiod and 75+5% relative humidity in insectary. After pupation, the pupae were collected from the soil and placed inside the oviposition chamber. After adult emergence, cotton soaked with 10% (w/v) sugar solution with a few drops of multivitamins was provided for adult feeding to increase the fecundity. Potted groundnut plant was kept inside an adult emergence cage for egg laying. After hatching the larvae were provided with tender castor leaves for feeding. The laboratory reared larvae were used for bioassay.

Antifeedant activity

Antifeedant activity of the fractions was studied using leaf disc no choice method. Fresh castor leaf discs of 4 cm diameter were punched using cork borer and were dipped in 125, 250, 500 and 1000 ppm concentrations. The leaf discs treated with acetone were used as negative control. In each plastic Petri dish (1.5 cm 6 9 cm), wet filter paper was placed to avoid early drying of the leaf discs and single third instar larva was introduced into each Petri dish. Progressive consumption of leaf by the treated and control larvae with 24 h was recorded using Leaf Area Meter (Delta-T Devices, Serial No. 15736 F 96, UK). Leaf area eaten by larvae in treatment was corrected from the negative control. Five replicates were maintained for each treatment with 10 larvae per replicate (total, n= 50). The experiment was conducted at laboratory conditions (27 ± 2°C) with 14:10 light and dark photoperiod and 75+5% relative humidity. The antifeedant activity was calculated using the following formula of Bentley et al. [19].

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Larvicidal activity

Larvicidal activity was studied using leaf disc no choice method. The larvae were fed on the castor leaf disc treated with different concentrations of 0.5, 1.0, 2.5 and 5.0% for crude extracts and 125, 250, 500 and 1000 ppm for fractions using leaf disc dip method. After 24 h of treatment, the larvae were continuously maintained on untreated fresh castor leaves. The diet was changed every 24 h. Larval mortality was recorded up to 96 h of treatment. Five replicates were maintained for each treatment with 10 larvae per replicate (total, n=50). Per cent mortality was calculated [20]. All the laboratory conditions were the same as in antifeedant study.

Pupicidal activity

The survived larvae were continuously fed with untreated castor leaf until they become pupae and adults. Pupal mortality was calculated by subtracting the number of emerging adults from the total number of pupae.

Statistical analysis

The antifeedant, larvicidal and pupicidal activities were subjected to one way analysis of variance (ANOVA). Significant differences between treatments were determined by DMRT F-test (p ≤ 0.05). LC50 value was calculated using Probit Analysis [21]. Linear regression analyses were performed for all dose–response experimental data.

Results

Crude extracts

Larvicidal activity: A. monophylla derived hexane, chloroform and ethyl acetate extracts showed larvicidal activity against S. litura. Table 1 shows larvicidal activity of different crude extracts of A. monophylla; hexane extract showed maximum larvicidal activity of 79.10% at 5.0% concentrations followed by chloroform and ethyl acetate extracts, which showed 43.77% and 22.88% larvicidal activity. Hexane extract at 0.5% showed 26.88% larvicidal activity. Lower concentration of chloroform and ethyl acetate extracts did not show larvicidal activity. They exhibited less than 50% larvicidal activity at all the tested concentrations. All the concentration of hexane showed statistically superior activity. Concentration dependent activity was noticed in all the three extracts.

Crude extracts Concentrations (%) LC50 R Coefficients
0.5 1.0 2.5 5.0
Hexane 26.88 ± 4.26b 43.99 ± 6.73b 58.22 ± 2.43c 79.10 ± 1.22c 2.01 0.98 y=9.33x+17.1
Chloroform 00 ± 00a 14.22 ± 5.29a 33.55 ± 6.25b 43.77 ± 4.12b 5.01 0.97 y=-14.77x+15.1
Ethyl acetate 00 ± 00a 08.22 ± 4.62a 16.44 ± 4.86a 22.88 ± 4.12a 7.80 0.92 y=-7.33 x+7.7
Within the column, means ± SD followed by the same letter do not differ significantly by using DMRT F test, (P ≤ 0.05); Effective concentration and complete regression equations.

Table 1: Larvicidal activity (%) and effective concentration of Atalantia monophylla crude extracts against Spodoptera litura.

Hexane extracts showed high correlation of R 0.98 and coefficient value of 9.33 ± 17.1 between concentration and larvicidal activity. Chloroform and ethyl acetate extract also showed high correlation of R 0.97 and 0.92 respectively. The least LC50 value of 2.01% was observed in hexane extracts. Ethyl acetate extract showed maximum LC50 value of 7.80%. Chloroform and ethyl acetate extract statistically similar at 0.5 and 1.0% concentrations.

Pupicidal activity: Hexane extract exhibited 100% pupicidal activity at 5.0% concentration against S. litura. Ethyl acetate extract showed 52.85% pupicidal activity at 5.0% concentrations. All the concentration of hexane extract statistically differed from chloroform and ethyl acetate extracts. Low concentration of chloroform and ethyl acetate extracts showed less than 10% pupicidal activity (Table 2).

Crude extracts Concentrations (%)
0.5 1.0 2.5 5.0
Hexane 45.71 ± 6.38c 69.33 ± 5.96c 85.00 ± 13.69d 100.00 ± 0.0d
Chloroform 08.22 ± 4.62b 23.88 ± 1.52b 47.71 ± 7.53c 70.66 ± 8.94c
Ethyl acetate 06.22 ± 5.69ab 20.55 ± 5.41b 32.50 ± 6.84b 52.85 ± 3.91b
Control 00.00a
Within the column, means ± SD followed by the same letter do not differ significantly by using DMRT F test, (P ≤ 0.05).

Table 2: Pupicidal activity (%) of Atalantia monophylla against Spodoptera litura.

Fractions

Antifeedant activity: Twelve fractions derived from hexane extract of A. monophylla showed concentration dependent antifeedant activity against S. litura. Maximum antifeedant activity of 85.31% was observed in fraction nine at 1000 ppm concentration followed by fraction 6 which showed 77.63%. The R value (0.92) exhibited good correlation and concentration dependent antifeedant activity with coefficient value (48.29 ± 9.2). Minimum antifeedant activity of 25.79% was observed in fraction 10 at 1000 ppm. Fractions 3, 5, 6 and 9th showed more than 70% antifeedant activity at 1000 ppm concentration (Table 3).

Fractions Concentration (ppm) R Coefficients
125 250 500 1000
1 36.06 ± 3.13ef 51.86 ± 5.94efg 59.04 ± 5.45e 64.19 ± 5.59de 0.89 y=29.89 x+9.2
2 26.91 ± 2.88cd 34.07 ± 3.22 46.75 ± 4.83cd 59.58 ± 3.33d 0.96 y=14.16x+11.1
3 31.41 ± 4.41def 48.63 ± 5.22def 58.59 ± 5.38e 70.88 ± 4.63ef 0.95 y=20.28x+12.8
4 26.56 ± 3.29bcd 34.73 ± 4.60c 40.67 ± 3.08c 51.04 ± 4.91c 0.92 y=18.41x+7.9
5 40.24 ± 3.65f 57.62 ± 5.09gh 67.31 ± 4.33f 72.79 ± 4.78f 0.92 y=32.66x+10.1
6 49.45 ± 5.46g 55.33 ± 2.32fg 70.61 ± 4.27fg 77.63 ± 2.78f 0.94 y=38.30 x+9.9
7 21.83 ± 4.51bcd 32.51 ± 3.14c 40.32 ± 1.80c 54.81 ± 4.42d 0.96 y=10.69x+10.7
8 37.09 ± 4.14f 46.36 ± 6.05de 50.17 ± 1.60d 59.90 ± 5.00cde 0.89 y=30.32x+7.2
9 58.27 ± 6.54h 65.36 ± 3.30h 76.21 ± 4.29g 85.31 ± 2.97g 0.93 y=48.29 x+9.2
10 17.28 ± 2.29b 20.71 ± 2.36b 21.32 ± 2.72b 25.79 ± 2.82b 0.73 y=14.74x+2.6
11 28.23 ± 6.64cde 41.63 ± 5.95cd 50.67 ± 4.12d 62.22 ± 4.75d 0.93 y=17.8x+11.2
12 20.98 ± 4.40bc 36.33 ± 2.72c 44.83 ± 3.14cd 60.14 ± 5.18d 0.96 y=9.07x+12.59
Control 3.77 ± 1.50a    
Within column, means ± SD followed by the same letter do not differ significantly by using DMRT-F test, (P ≤ 0.05); complete regression equations

Table 3: Antifeedant activity (%) of Atalantia monophylla fractions against Spodoptera litura.

Larvicidal activity: Table 4 shows the larvicidal activity of different fractions from hexane extracts of A. monophylla. Maximum larvicidal activity of 83.55% was observed in fraction 9 at 1000 ppm concentration, also it exhibited least LC50 value of 340.27 ppm and higher regression coefficient value of y=6.22x+20.44 for larvicidal activity against S. litura than other fractions. Fractions 2 and 7 did not show larvicidal activity at all the concentrations. Except 9th and 5th fractions, all other fractions exhibited less than 50% larvicidal activity. Minimum larvicidal activity was noticed in fraction 12 but maximum LC50 was observed in fraction 10.

Fractions Concentration(ppm) R Coefficients LC50(ppm)
125 250 500 1000
1 00.00 ± 0.0a 16.44 ± 4.86c 31.33 ± 1.82d 39.55 ± 3.97e 0.89 y=-8.55x+7.71 1476.64
2 00.00 ± 0.0a 00.00 ± 0.0a 00.00 ± 0.0a 00.00 ± 0.0a - - -
3 00.00 ± 0.0a 06.44 ± 5.89ab 12.88 ± 5.24b 23.55 ± 5.57cd 0.97 y=-11.55x+13.4 1084.36
4 00.00 ± 0.0a 08.22 ± 4.62b 22.88 ± 4.12c 26.88 ± 4.26d 0.94 y=-9.33x+9.5 1368.21
5 10.44 ± 0.60b 24.88 ± 4.75d 35.55 ± 6.42d 43.77 ± 4.12e 0.94 y=1.00x+11.1 1063.73
6 14.44 ± 5.09b 26.88 ± 4.26d 45.77 ± 4.26e 62.66 ± 3.64f 0.98 y=-3.44x+16.4 710.94
7 00.00 ± 0.0a 00.00 ± 0.0a 00.00 ± 0.0a 00.00 ± 0.0a - - -
8 00.00 ± 0.0a 08.44 ± 4.75b 14.44 ± 5.09b 20.88 ± 1.21bcd 0.92 y=-6.22x+6.9 1639.77
9 25.11 ± 6.16c 45.77 ± 4.26e 74.88 ± 6.16f 83.55 ± 4.86g 0.96 y=6.22x+20.44 340.27
10 00.00 ± 0.0a 08.22 ± 4.62b 10.22 ± 0.49b 16.44 ± 4.86bc 0.90 y=-4.11x+5.1 1927.89
11 00.00 ± 0.0a 10.44 ± 0.60bc 12.44 ± 4.26b 22.88 ± 4.12cd 0.92 y=-6.2x+7.1 1598.43
12 00.00 ± 0.0a 00.00 ± 0.0a 08.22 ± 4.62b 14.44 ± 5.09b 0.85 y=-7.2x+5.6 1609.20
Within column, means ± SD followed by the same letter do not differ significantly by using DMRT-F test, (P ≤ 0.05); Effective concentration and complete regression equations.

Table 4: Larvicidal activity (%) of Atalantia monophylla fractions against Spodoptera litura.

Pupicidal activity: Maximum pupicidal activity of 100% was noticed in fraction 9 at 500 and 1000 ppm concentrations followed by fraction 6 which exhibited 80 and 100% pupicidal activity at 500 and 1000 ppm concentrations. Fractions 2, 7 and 10 did not show any pupicidal activity at all the tested concentrations. More than 50% pupicidal activity was noticed in fraction 3, 5, 6 and 9 at 1000 ppm concentration (Table 5).

Fraction Concentration (ppm)
125 250 500 1000
1 10.66 ± 0.60b 16.54 ± 4.95b 21.33 ± 6.91c 28.33 ± 4.56b
2 00.00 ± 0.0a 00.00 ± 0.0a 00.00 ± 0.0a 00.00 ± 0.0a
3 20.88 ± 1.21c 27.5 ± 5.59c 45.71 ± 3.91d 55.33 ± 7.67c
4 08.22 ± 4.62b 15.83 ± 5.85b 24.28 ± 5.86c 31.42 ± 6.38b
5 20.83 ± 4.81c 37.85 ± 5.84d 45.14 ± 4.58d 62.66 ± 3.65d
6 34.44 ± 6.83d 45.71 ± 6.38e 80.66 ± 1.49e 100.00 ±0.0e
7 00.00 ± 0.0a 00.00 ± 0.0a 00.00 ± 0.0a 00.00 ± 0.0a
8 00.00 ± 0.0a 00.00 ± 0.0a 12.22 ± 0.62b 26.42 ± 1.95b
9 51.07 ± 8.71e 78.66 ± 6.91f 100.00 ± 0.0f 100.00 ± 0.0e
10 00.00 ± 0.0a 00.00 ± 0.0a 00.00 ± 0.0a 00.00 ± 0.0a
11 00.00 ± 0.0a 00.00 ± 0.0a 11.94 ± 0.76b 27.14 ± 1.95b
12 00.00 ± 0.0a 00.00 ± 0.0a 09.16 ± 5.15b 24.44 ± 1.24b
Control 00.00 ± 0.0a
Within column, means ± SD followed by the same letter do not differ significantly by using DMRT F test, (P ≤ 0.05).

Table 5: Pupicidal activity (%) of Atalantia monophylla fractions against Spodoptera litura.

Discussion

In the present study hexane extract of A. monophylla exhibited maximum larvicidal activity against S. litura. The present findings coincide with the findings of Muthu et al. [15] who noticed that hexane extract of A. monophylla showed 85.33% larvicidal activity at 5.0% concentration against E. vittella. Extracts from Sonchus oleraceus (Asteraceae), Raphanus sativus L. and Brassica nigra L. (Brassicaceae) exhibited larvicidal activity against S. littoralis [22]. Abbasipour et al. [23] reported that Peganum harmala L. (Nitrariaceae) seed extract showed 100% insecticidal activity against diamondback moth, Plutella xylostella at 40 mg/ml concentration.

In this study A. monophylla derived hexane extracts exhibited LC50 value of 2.01% for larvicidal activity against S. litura. The present findings support the findings of Baskar et al. [14] who reported that hexane extract of A. monophylla exhibited LC50 value of 2.46% concentration for larvicidal activity against H. armigera. Leaf methanol extract of Jatropha gossypiifolia L. (Euphorbiaceae) showed least LC50 value of 19.75 mg/ml concentration against S. litura [24].

In this study hexane extract of A. monophylla exhibited 100% pupicidal activity against S. litura at 5.0% concentration. Present findings coincide with the findings of Baskar et al. [14] who reported that hexane extract kill 100% pupae of H. armigera at 5.0% concentration. Muthu et al. [15] reported that hexane extract of A. monophylla reduced the adult emergence of E. vittella in a dose dependent manner. Leaves of Ayapana triplinervis (Vahl) R. M. King & H. Rob (Syn. Eupatorium triplinerve) (Asteraceae) derived aqueous extract showed pupicidal activity against S. litura [25]. Abbasipour et al. [23] reported that P. harmala seed extract completely inhibit the adult emergence of P. xylostella at 40 and 30 mg/ml concentrations.

In this study fractions from hexane extract of A. monophylla exhibited antifeedant activity against S. litura. Among them 9th fraction exhibited 85.31% antifeedant activity at 1000 ppm concentration. The present findings coincide with the findings of Baskar et al. [14] who reported that hexane extract derived fractions showed 87.28% of antifeedant activity against H. armigera at 1000 ppm concentration. Hexane and methanol fractions from leaves of Glycosmis arborea (Roxb.) (Rutaceae) showed antifeedant activity against S. litura [26]. You-Zhi et al. [27] reported that magnificol, millettocalyxin C and Isoloncharpin derived from Derris cavaleriei Gagnepain, Notul. Syst. (Paris) (Fabaceae) showed antifeedant activity against P. xylostella.

In this study A. monophylla derived fractions showed larvicdal activity against S. litura. Maximum larvicidal activity of 83.55% was observed in 9th fraction. This result corroborates with the findings of Ramya et al. [28] who reported that Catharanthus roseus L (G) Don. (Apocynaceae) leaves derived crude extracts from methanol, petroleum, and fractions from methanol and ethyl acetate evaluated against H. armigera, all extracts exhibited moderate larvicidal effects. Maximum larvicidal activity was noticed in ethyl acetate fraction. In the present study, fraction 9 showed least LC50 value of 340.27 ppm for larvicidal activity. Similarly, Baskar et al. [14] reported that fraction nine from hexane extract of A. monophylla showed least LC50 value of 384.57 ppm for larvicidal activity against H. armigera. Methanol extract, its fraction and isolated compound furocoumarin and quinolone alkaloid from Ruta chalepensis L. (Rutaceae) exhibited LC50 values of 2.42, 0.89, 1.59 and 1.21 mg/ml for larvicidal activity against S. littoralis [29].

Hexane derived fractions from A. monophylla showed pupicidal activity against S. litura. Maximum pupicidal activity of 100% was observed in 9th fraction at 1000 ppm concentration. The results support with the earlier findings of Baskar et al. [14] who reported that fractions from hexane extract of A. monophylla showed pupicidal activity against H. armigera. Moringa oil (Moringaceae) and saponifiable oil showed pupicidal activity against S. frugiperda [30]. In the present study, different fractions showed pupicidal activity at concentration dependent manner. The present findings are in agreement with the findings of Huang et al. [8] who reported that Pogostone from Pogostemon cablin (Blanco) Benth. (Lamiaceae) exhibited pupicidal activity against S. litura and S. exigua.

Conclusion

Hexane extract and their fractions from A. monophylla showed antifeedant, larvicidal and pupicidal activities against S. litura. Dose depend activity was noticed in all the bioassays. Linear regression results support the dose depend activity. The hexane derived 9th fraction could be further fractionated and isolate the active principle(s) responsible for the activity. Further, hexane extract of A. monophylla could be used to develop a new formulation to manage the agriculturally important pests.

Conflict of Interest Statement

We declare that we have no conflict of interest.

Acknowledgement

The authors thank the Department of Science and Technology (Ref. no.: SR/ SO/AS-03/2004), New Delhi for financial support.

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Citation: Baskar K, Muthu C, Ignacimuthu S (2015) Bio Efficacy of Atalantia monophylla (L) Correa (Rutaceae) against Spodoptera litura Fabricius (Lepidoptera: Noctuidae). Entomol Ornithol Herpetol 4:145.

Copyright: © 2015 Baskar K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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