Current Synthetic and Systems Biology
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Catalytical Enzymes: Classification and Biological Applications

Firouse Ghadim^{* *}Correspondence: Firouse Ghadim, Department of Cell Biology, John Cabot University, Rome, Italy, Email:

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Description

Enzymes are biomolecules that serve as nature's remarkable catalysts. These specialized proteins play a crucial role in countless biological processes, ranging from digestion and metabolism to DNA replication and cellular signaling. By speeding up chemical reactions and lowering the activation energy required, enzymes enable life-sustaining processes to occur at body temperature and within the constraints of living systems.

This article explores the structure, function, and applications of enzymes, highlighting their significance in various industries and their potential for future advancements [1].

Structure and function of enzymes

Enzymes possess a specific three-dimensional structure that allows them to carry out their catalytic function. Composed of long chains of amino acids, enzymes fold into unique shapes that create an active site which is a region where substrate molecules bind and undergo chemical transformations. The lock-and-key or induced fit model describes the interaction between enzymes and substrates, emphasizing the complementary fit required for catalysis [2-4].

Enzymes work by lowering the activation energy of a reaction, enabling it to proceed more rapidly. They achieve this by stabilizing the transition state of the reaction, facilitating the formation of product molecules. Importantly, enzymes themselves remain unchanged after the reaction, allowing them to participate in multiple cycles.

Classification of enzymes

Enzymes are classified into several categories based on the type of reaction they catalyse. Oxidoreductases, for example, facilitate oxidation-reduction reactions, while hydrolases break down molecules through hydrolysis. Transferases, isomerases, and ligases are other classes of enzymes that perform specific types of chemical modifications or joining reactions [5,6].

Biological applications of enzymes

Enzymes find essential biological applications across various fields due to their catalytic properties. In digestion, enzymes like amylase, lipase, and protease aid in breaking down food molecules for absorption. They are vital in metabolic pathways, such as glycolysis and the citric acid cycle, enabling energy production. Enzymes also play a role in DNA replication, repair, and protein synthesis. In medicine, enzymes are employed for diagnostic tests, pharmaceutical production, and drug design [7].

The food and beverage industry utilizes enzymes to enhance food quality and nutritional content. Additionally, enzymes have applications in environmental processes, biotechnology, and as valuable research tools. Their versatility and specificity make enzymes indispensable for advancing our understanding of biology and addressing various challenges in medicine, industry, and the environment [8,9].

Future perspectives

Enzymes play a pivotal role in biology, and their future perspectives are promising. As biotechnology advances, enzymes are becoming increasingly significant in various fields. In medicine, enzyme therapies hold potential for treating genetic disorders and immune-related diseases. Enzymes also play a crucial role in sustainable industries, aiding in biofuel production, waste management, and bioremediation. In agriculture, enzymes can enhance crop yield and promote sustainable practices. Additionally, enzyme engineering and synthetic biology are revolutionizing enzyme production, leading to cost-effective and efficient processes. As researchers are understanding enzyme function and structure, novel applications may emerge, such as targeted drug delivery and nanotechnology. Furthermore, enzymes are instrumental in diagnostic tools, facilitating early disease detection. Overall, the continuous exploration of enzymes will drive innovation in biotechnology, contributing to breakthroughs that address global challenges and improve human life [10].

References

1. Endo A, Kuroda M, Tsujita Y. ML-236A, ML-236B, and ML-236C, new inhibitors of cholesterogenesis produced by Penicillium citrinum. J Antibiot. 1976; 29(12):1346-1348.

   [Crossref] [Google Scholar]

2. Kurumbail RG, Kiefer JR, Marnett LJ. Cyclooxygenase enzymes: Catalysis and inhibition. Curr Opin Struct Biol. 2001;11(6):752-760.

   [Crossref] [Google Scholar]

3. Dunaway-Mariano D. Enzyme function discovery. Structure. 2008;16(11):1599-1600.

   [Crossref] [Google Scholar]

4. Chen LH, Kenyon GL, Curtin F, Harayama S, Bembenek ME, Hajipour GH, Whitman CP. 4-Oxalocrotonate tautomerase, an enzyme composed of 62 amino acid residues per monomer. J Biol Chem 1992;267(25):17716-17721.

   [Crossref] [Google Scholar]

5. Hubscher U, Shevelev IV. The 3'5'exonucleases. Nat Rev Mol Cell Biol. 2002;3(5):364-376.

   [Crossref] [Google Scholar]

6. Zenkin N, Yuzenkova Y, Severinov K. Transcript-assisted transcriptional proofreading. Science. 2006;313(5786):518-520.

   [Crossref] [Google Scholar]

7. Bajpai P. Application of enzymes in the pulp and paper industry. Biotechnology progress. 1999; 15(2):147-157.

   [Crossref] [Google Scholar]

8. Ramanathan A, Savol A, Burger V, Chennubhotla CS, Agarwal PK. Protein conformational populations and functionally relevant substates. Accounts of chemical research. 2014;47(1):149-156.

   [Crossref] [Google Scholar]

9. Jorgensen WL. Rusting of the lock and key model for protein-ligand binding. Science. 1991;254(5034):954-955.

   [Crossref] [Google Scholar]

10. Fieker A, Philpott J, Armand M. Enzyme replacement therapy for pancreatic insufficiency: present and future. Clin Exp Gastroenterol. 2011;4:55-73.

    [Crossref] [Google Scholar]