Medicinal & Aromatic Plants

Medicinal & Aromatic Plants
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Research Article - (2016) Volume 5, Issue 3

Chemical Composition and Antibacterial Activity of Volatile Oil of Sequoia sempervirens (Lamb.) Grown in Egypt

Taha KF and Shakour ZTA*
Laboratory of Phytochemistry, National Organization for Drug Control and Research, Cairo, Egypt
*Corresponding Author: Shakour ZTA, Laboratory of Phytochemistry, National Organization for Drug Control and Research, Cairo 11364, Egypt, Tel: 01274885786 Email:

Abstract

The hydrodistilled essential oil of leaves of Sequoia sempervirens (Lamb.) belonging to family Cupressaceae was analyzed by GC/MS. Thirty six compounds were identified representing 95.62% of the total oil containing α-phellandrene (29.60%), dl-limonene (15.60%), α-pinene (8.65%) and terpinene-4-ol (3.5%) as major components. The studied essential oil showed a dose-dependent antioxidant activity using 2, 2-diphenyl-1-picrylhydrazyl assay (IC50: 32.2 μg/mL). The antimicrobial activity of S. sempervirens leaves oil was screened against Gram-positive and Gram-negative bacteria and fungi. The strongest antibacterial effects of oil were on Bacillus subtilis, Trichophyton mentagrophytes and Staphylococcus aureus. S. sempervirens oil showed potent cytotoxic activity against the tested carcinoma cell lines; hepatocellular (HEPG2), colon adenocarcinoma (HCT-116) and breast (MCF7) comparing to doxorubicin.

Keywords: S. sempervirens; GC/MS; Antioxidant; Antimicrobial; Cytotoxic

Introduction

Cupressaceae family is dioecious or monoecious shrubs or trees containing about 18 genera and 140 species, in which the leaves are in opposite pairs, addressed and scale-like, or needle-like, the cones are usually small, globose too long. The scales of the cones have no spine tips [1]. They distributed in the northern temperate zone, with outlying species in tropical mountains and in temperate America. The family is notable for including the largest, tallest, and stoutest individual trees in the world (Giant Sequoia), and also the second longest lived species in the world (Coast Redwood) [1].

Sequoia is a genus of redwood coniferous trees in the subfamily Sequoioideae of the family Cupressaceae. The only extant species of the genus is Sequoia sempervirens in the Northern California coastal forests ecoregion of Northern California and Southwestern Oregon in the United States [2]. The two other genera, Sequoiadendron and Metasequoia, in the subfamily Sequoioideae are closely related to Sequoia [3]. It is a member of the cypress (Cupressaceae) family and like most cypresses is evergreen, as indicated by its species name sempervirens, meaning ‘always green’. It is one of the tallest trees in the garden [4]. Through evaluation of the state of trees and shrubs of Egypt, it was revealed that some nine indigenous species are threatened with extinction in natural Egyptian Environs of which Sequoia sempervirens is one tree in El Orman Garden [5]. Chemical constituents of S. sempervirens oil were seldom reported before; in Egypt, no attention has been paid to evaluate the volatile oil, although it has many commercial and medicinal uses even it was possible to propagate S. sempervirens tree through tissue culture techniques [6].

The objectives of this work are to demonstrate the antioxidant, antimicrobial and a cytotoxic activity in correlation to phytochemical constituents of oil from S. sempervirens (Lamb.) leaves.

Materials and Methods

Plant material

Sequoia sempervirens (D. Don.) leaves were collected during October and May, respectively, from El Orman Garden, Giza, Egypt (2012). The collected plant materials were botanically authenticated by Prof. Dr. Monir Mohamed abd Elghany, The Herbarium, Botany department, Faculty of Science, Cairo University, Egypt, also Voucher specimen of the authenticated plant was deposited at Laboratory of phytochemistry, National Organization for Drug Control and Research. Cairo, Egypt.

Preparation of the essential oil

The fresh leaves of S. sempervirens (Lamb.) were subjected to hydro distillation [7]. The essential oil obtained was dried over anhydrous sodium sulphate and kept in a refrigerator for analysis. The percentage of the oil was calculated on fresh weight bases.

Analysis of the essential oil

GC/MS analysis of the essential oil was carried out on an Agilent 6890 equipped with a mass spectrometric detector (MSD), model Agilent 5973, equipped with an HP-5MS column (30 m × 0.25 mm, 0.25 μm); programming from 80 (3 min) to 260°C at 8°C/min, 10 min hold; carrier gas, helium; flow rate, 1.0 ml/min; injection in split mode (60.1); injector and detector temperatures 225°C and 300°C, respectively. The EIMS mode at 70 eV; electron multiplier, 2500 V; ion source temperature, 250°C; mass spectra data were acquired in the scan mode in the m/z range 50 to 700. The essential oil components were identified by comparing their mass fragmentation patterns with those of the available reference [8]. In addition, qualitative analysis was carried out by using internal normalization method (peak area measurement) and compound identification was confirmed by electronic Wiley and NIST mass spectral data base. The retention indices (RI) of the volatile oil components were determined relative to the retention times of series of hydrocarbons. Results are given in Tables 2 and 3.

Physical characters S. sempervirens oil
Percentage yield (%w/v) 0.68
Color Pale yellow
Odor Characteristic, strongly aromatic
RI 1.4256
Sp. gr. 0.96

Table 1: Physical characters of leaf essential oil of S. sempervirens (Lamb.).

No. Compound % K.I RTT*
1. α-pinene 8.65 939 0.49
2. Sabinene 0.75 975 0.86
3. b-Myrcene 0.64 985 0.98
4. α-Phellandrene 29.60 1003 1
5. p-Cymene 1.20 1023 1.03
6. dl-limonene 15.60 1029 1.06
7. cis-β-Ocimene 1.35 1040 1.07
8. trans-β-Ocimene 0.75 1052 1.09
9. m-Cymene 1.45 1061 1.15
10. γ-Terpinene 1.82 1062 1.16
11. Borneol 0.73 1088 1.19
12. Fenchone 0.85 1091 1.24
13. Terpinolene 1.32 1143 1.33
14. Terpinene-4-ol 4.35 1178 1.38
15. p-Cymen-8-ol 0.73 1188 1.39
16. α-Terpineol 0.26 1189 1.43
17. cis-Piperitol 0.65 1200 1.49
18. trans-Piperitol 0.92 1210 1.54
19. β-Citronellol 1.12 1230 1.58
20. Geraniol 0.34 1253 1.62
21. Thymol 0.69 1290 1.68
22. Carvacrol 1.21 1298 1.71
23. α-Terpinyl acetate 3.65 1345 1.76
24. Thymol acetate 1.45 1350 1.82
25. Eugenol 0.43 1392 1.84
26. β-Caryophyllene 0.53 1419 1.87
27. trans-α-Bergamotene 0.26 1434 1.91
28. Germacrene D 2.54 1469 1,93
29. α-Humulene 1.08 1454 1.96
30. Trans-β-farnesene 0.73 1457 2.21
31. E,E- α- Farnesene 0.89 1506 2.45
32. β-Germacrene 4.87 1561 2.58
33. Spathulenol 0.75 1581 2.63
34. Citronellylpentanoate 1.06 1629 2.71
35. α–Cadinol 0.65 1654 2.79
36. Phytol 1.53 1940 2.84
KI=Kovat's index; RRT*=Relative retention time; α-Phellandrene=1 of RT=10.05.

Table 2: Chemical composition of leaf essential oil of S. sempervirens (Lamb.).

Oil constituents Total identified (%)
% of identified component 95.4
% of unidentified components 4.6
Monoterpene hydrocarbons 63.39
Sesquiterpene hydrocarbons 10.64
Other hydrocarbons -
Total hydrocarbons 74.03
Oxygenated monoterpenes 17.28
Oxygenated sesquiterpenes 2.13
Other oxygenated constituents 1.96
Total oxygenated compounds 21.37
Total monoterpenes 80.67
Total sesquiterpenes 12.77
Major constituent b- phellandrene

Table 3: The calculated percentage of different classes of components of leaves essential oil of S. sempervirens (Lamb.).

Determination of antioxidant activity by the DPPH radical scavenging assay

The free radical scavenging and antioxidant activities of the oil against the stable free radical DPPH were measured. Briefly; 10 μL of different concentrations of essential oils (5-35 μg/ml in DMSO) was added to 190 μL of ethanolic solution of DPPH. After 30 minutes of incubation at room temperature in the dark, the absorbance of the resulting mixture at 517 nm using Spectra max 340 USA (molecular devices). Appropriate blank (DMSO) and standard (Ascorbic acid in distilled water) solutions were prepared and run simultaneously. Ascorbic acid was used as reference compound. The assay was carried out in triplicate. The percentage inhibition (I %) for each concentration was calculated by using the absorbance (A) values according to the following formula:

I%=[(Ablank-Aoil)/Ablank] × 100

Where: Ablank is the absorbance of blank solution and Aoil is the absorbance of the oil. The dose-response curve was plotted and IC50 value for the oil was calculated [9].

Antimicrobial screening

A series of bacterial and fungal strains available in stock culture of the Regional Center for Mycology and Biotechnology Antimicrobial Unite (RCMB), Cairo, Egypt, were used for antibiotic sensitivity testing comprising: Gram-positive Bacteria [Staphylococcus aureus (RCMB 010015) Bacillus subtilis (RCMB010016) Lactobacillus acidophilus (RCMB 010023)], Gram-negative Bacteria [Salmonella typhimurium (RCMB 010076) Escherichia coli (RCMB 010085) Klebsiella pneumoniae (RCMB 0010082) Shigella flexneri (RCMB 0100876) Pseudomonas aeruginosa (RCMB 010043) Proteous vulgaris (RCMB 010062)], and four Fungi [Aspergillus flavus (RCMB 02554), Aspergillus niger (RCMB 05033), Geotricum candidum (RCMB 05096), Trichophyton mentagrophytes (RCMB 09254)]. The previously prepared essential oil was diluted 1/3 v/v in dimethyl sulphoxide (E-Merck), 30 μl (containing 10 μl of pure oil) were used in the test. Dimethyl sulphoxide (50 μl) was used as a negative control. The agar diffusion method [10] was applied using trypticase soy agar (Difco) medium inoculated with the bacterial or fungal suspension of the test organisms. Discs 5 mm in diameter were impregnated with the oils or the control. Then the discs were placed onto the surface of the culture medium. Discs of ceftriaxon and clotrimazole were used as standard antibacterial and antifungal agent, respectively. The plates were incubated at 35- 37°C for 24-48 hours in case of bacteria, 25°C for 48 hours in case of filamentous fungi, while yeasts were incubated at 30°C for 24-48 hours. After incubation, the diameters of inhibition zones were recorded in millimeters and the results were compiled in Table 4. The minimum inhibitory concentrations (MIC) of S. sempervirens oil against the tested microorganisms were also determined by micro dilution method [11] results are recorded in Table 5.

Tested microorganisms Essential oil Standard
Gram-Positive Bacteria
Staphylococcus aureus (RCMB 010015) Bacillus subtilis (RCMB010016)
Lactobacillus acidophilus (RCMB 010023)
15.6 ± 1.16
17.2 ± 2.24
15.1 ± 1.32
Ampicillin
20.6 ± 1.12
25.2 ± 2.18
26.4 ± 2.34
Gram-negative Bacteria
Salmonella typhimurium (RCMB 010076
Escherichia coli (RCMB 010085 )
Klebsiella pneumoniae (RCMB 0010082) Shigella flexneri (RCMB 0100876) Pseudomonas aeruginosa (RCMB 010043)
Proteous vulgaris (RCMB 010062)
13.4 ± 1.39
12.5 ± 1.16
11.1 ± 2.16
13.4 ± 1.68
14.3 ± 1.19
12.8 ± 1.23
Gentamycin
19.3 ± 1.19
23.2 ± 1.32
26.1 ± 2.16
23.8 ± 1.23
17.7 ± 1.22
25.4 ± 0.97
Fungi
Aspergillus flavus (RCMB 02554)
Aspergillus niger (RCMB 05033)
Geotricum candidum (RCMB 05096)
Trichophyton mentagrophytes (RCMB 09254)
NA
13.0 ± 1.22
12.2 ± 1.13
16.6  ± 1.17
Amphotericin B
24.6 ± 2.10
21.8 ± 1.12
26.4 ± 1.20
25.4 ± 2.16
The test was done using the agar disc diffusion technique, Well diameter 6.0 mm, (100 μl was tested), RCMB: Regional Center for Mycology and Biotechnology Antimicrobial unit test organisms. *NA: No activity, data are expressed in the form of mean ± SD. A value of P<0.05 was accepted as significant

Table 4: Antibacterial activity of leaves essential oil of S. sempervirens (Lamb.) against the ested microorganisms.

Tested microorganisms MIC (μl/mL)
Staphylococcus aureus (RCMB 010027) 4.2
Staphylococcus epidermidis (RCMB 010024) 6.1
Streptococcus pyogenes (RCMB 010015) 5.3
Neisseria gonorrhoeae (RCMB 010076 7.3
Proteous vulgaris (RCMB 010085 ) 7.8
Klebsiella pneumoniae (RCMB 0010093) 8.4
Shigella flexneri (RCMB 0100542) 7.1
Pseudomonas aeruginosa (RCMB 010043 6.9
Escherichia coli (RCMB 010056) 7.6
Aspergillus fumigatus (RCMB 02564) ND
Candida albicans (RCMB 05035) 8.2
Geotricum candidum (RCMB 05096) 9.9
Trichophyton mentagrophytes (RCMB 0925) 6.2
ND; Not done, as essential oil (s) has no antimicrobial activity on this microorganism.

Table 5: Minimum inhibitory concentrations (MIC) of essential oil of S. sempervirens (Lamb.) leaves against the tested microorganisms.

Cytotoxic activity

The cytotoxic activity of the oil under investigation was tested against hepatocellular (HEPG2), Colon adenocarcinoma (HCT-116) and breast (MCF7) carcinoma cell lines, obtained from National Cancer Institute, Kasr El Ainy, Cairo, Egypt, using Sulphorhodamine B assay (SRB) according to the method of [12]. The oil was dissolved in saline solution in a concentration of 100 μg/100 μl, tween 80 was used to help dissolution of insoluble materials. The obtained IC50 were compared with that of doxorubicin as reference drug; results are recorded in Table 7.

Conc. (μg/ml) Ascorbic acid Essential oil
35 80.12 ± 0.55 75 ± 0.47
30 78 ± 0.45 68 ± 0.32
25 76 ± 0.31 54 ± 0.25
20 71 ± 0.26 37 ± 0.41
15 68 ± 0.35 25 ± 0.21
10 28 ± 0.17 19 ± 0.45
5 14 ± 0.08 13 ± 0.15
0 0 0
IC50 16.9 32.2
Data are expressed in the form of mean ± SD. A value of P<0.05 was accepted as significant.

Table 6: In vitro DPPH radical scavenging activity of essential oil of S. sempervirens (Lamb.) leaves.

S. sempervirens oil and drug IC50μl/ml
HEPG2 HCT-116 MCF7
Essential oil 7.567 8.835 9.782
Doxorubicin 0.6 0.85 0.7

Table 7: Results of cytotoxic activity of of leaf essential oil of S. sempervirens (Lamb.).

Statistical analysis

The results were subjected to statistical analysis. Values were reported as Mean ± SD. The data were analysed using Student’s t-test to test for differences between treatment and control where a value of P<0.05 was accepted as significant.

Results and Discussion

Physical characters of the essential oil

The color, odor, yield, refractive index (20°C) and the specific gravity (20°C) of the hydro distilled oil were examined. The essential oil prepared from S. sempervirens leaves was obtained as pale yellow oil with characteristic, strongly aromatic odor. The percentage yield of the oil was 0.68% v/w (calculated on fresh weight basis), Table 1.

GC/MS analysis of the essential oils

Thirty-six constituents in the essential oil of S. sempervirens leaves were identified corresponding to 95.4% of the total oil. The results revealed that terpenoids (including saturated hydrocarbon, monoterpenoids, sesquiterpenoids and oxygenated ones) in the oil were predominant. The main constituents of the essential oil were α-phellandrene (29.60%), dl-limonene (15.60%), α-pinene (8.65%), β-germacrene (4.87%), respectively. In general the percent of hydrocarbons (74.03%) is higher than the percent of oxygenated compounds 21.37% in the oil sample under investigation, also the percent of monoterpenoid 80.67% compounds is higher than that of sesquiterpenes 12.77%. The percentages of total hydrocarbons, monoterpene and sesquiterpene hydrocarbons as well as the percentages of total oxygenated compounds, oxygenated monoterpenes and sesquiterpenes were calculated and are compiled in Tables 2 and 3. This result agreed with the previous results submitted by Sefidkon et al. [13] who reported that the main components of oil from S. sempervirens leaves were b- phellandrene and limonene (13.30%), a- pinene (6.83%), terpinene -4-o1 (6.47%), g-teroubebe (5.44%) and germacrene B (4.17%).

Antimicrobial activity

In the present study, the antibacterial activity of S. sempervirens leaves essential oil was tested by the disc diffusion method against nine bacterial species. Results showed that the leaves essential oil, at concentration of 20 μL/disc, gave inhibition activities (more than 6 mm diameter hollow zones) against all tested Gram-positive species and all tested Gram-negative bacteria species, they were all sensitive to the essential oil comparing to Ampicillin and Gentamycin, respectively. Antifungal activity of the essential oil of leaves of S. sempervirens was tested against four fungal species. All tested fungal species were susceptible to the discs impregnated with the tested essential oil with the exception of Aspergillus flavus which was totally resistant to leaves essential oil (Table 4). Although the essential oil of S. sempervirens had better antibacterial activity against Gram-positive species, it showed broad-spectrum antibacterial and antifungal action as well. Accordingly, the essential oil of the leaves should be further investigated to evaluate its efficiency and safety in clinical practice. The MIC of leaves essential oil against the tested microorganism is presented in Table 5. The results revealed variability in the inhibitory concentrations of the essential oil against each microorganism, in the range (concentrations) from 4.2 to 9.9 μl/mL. The lowest variation was observed for essential oil on Staphylococcus aureus followed by Streptococcus pyogenes and Staphylococcus epidermidis. Previously, the volatile oil of S. sempervirens showed antifungul activity against Pleuroplaconema sp. [14].

Earlier papers on the antibacterial activities of α-phellandrene, limonene, α- pinene, and β-caryophyllene have shown that they have varying degrees of growth inhibitory effects against some bacteria [15- 17]. The current study shows that the antimicrobial activity of the oils from S. sempervirens leaves could, in part, be associated with its major components (α-Phellandrene,dl-limonene and α-pinene).

Antioxidant activity

Antioxidant potential of essential oil of S. sempervirens leaves was determined as its DPPH radical scavenging ability, Table 6. The essential oil exhibited higher free radical scavenging activity (75 ± 0.47%) at a conc. of 35 μg/ml with IC50 value equal to 32.2 μg/ml. Studies by Bajpai et al. [18]. evaluated the antioxidant activity of the essential oil obtained from of Metasequioa glyptostroboides Miki leaves, a species of the same family verified the free radical scavenging activity of the oil was found to be 11.32 μg/ml. The antioxidant activity of essential oil can be attributed to the synergistic activities of multiform unsaturated compounds such as α-phellandrene, limonene and α-pinene which reported to have antioxidant activities [19,20].

Anticancer activity

The essential oil of S. sempervirens leaves showed antitumor activities against all tested cell lines (IC50 Values <10 μg/ml). The IC50 values were 7.567, 8.835 and 9.782 μg /ml for HEPG2, HCT-116 and MCF7 respectively. The cytotoxic activity of the essential oil of S. sempervirens leaves could be attributed to its hydrocarbon contents [21-23]. Other studies has verified that α-Phellandrene, dl-limonene, α-pinene, have been described as cytotoxic compounds against different cell lines [24-27]. Regarding that bioactive cytotoxic compounds have been found in the essential oil from S. sempervirens leaves, it is expectable to observe cytotoxic activity against the examined cell lines In addition, the components with lower concentrations, such as α- Farnesene, β-Germacrene α- humulene and β-caryophyllene, may also be contributing to the cytotoxic activity of the oil. Therefore, the synergistic effects of the major and minor components of the essential oils should be taken into consideration to account for the oil biological activity [28-30].

Conclusion

The antimicrobial, antioxidant and cytotoxic activities of the essential oils from many plants are of great interest to both the academe and the pharmaceutical, cosmetic, and food industries because of their possible use as natural additives to replace synthetic compounds. For the first time, we reported that the essential oil of S. sempervirens leaves exhibits antioxidant, cytotoxic activities and successfully inhibits the growth of different microorganisms. The results obtained in this study show that the essential oil of S. sempervirens leaves may be a new potential source of natural antimicrobial, antioxidant and cytotoxic agents so they could be used in pharmaceutical formulations. However, further studies need to be conducted to understand the mechanism of the activity and obtain more information on the safety and toxicity of the oil. In general, we recommend that a further study under the in vivo conditions to further elaborate the antimicrobial, antioxidant and cytotoxic principles of S. sempervirens essential oil for various useful applications. The investigated essential oil may be used for the preservation of processed foods as well as pharmaceutical and natural therapies for the treatment of infectious diseases in humans and plants.

Acknowledgements

The authors thank the Regional Center for Mycology and Biotechnology Antimicrobial Unite (RCMB), Cairo, Egypt for supplying microbial stains for performing the antimicrobial study.

Declaration of Interest

The authors declare that there is no conflict of interest. The authors alone are responsible for the content of the paper.

References

  1. Farjon A (2005) Monograph of Cupressaceae and Sciadopitys. Royal Botanic Gardens, Kew.
  2. Ahuja MR, Neale DB (2002) Origins of polyploidy in coast redwood (Sequoia sempervirens) and relationship of coast redwood to other genera of Taxodiaceae. Silvae Genetica 51: 93-100.
  3. Deborah LR (2000) Genotypic diversity and clone size in old-growth populations of coast redwood (Sequoia sempervirens). Canadian Journal of Botany 78: 1408-1419.
  4. Farjon A,Schmid R (2011) Sequoia sempervirens. IUCN Red List of Threatened Species. Version 2013.2. International Union for Conservation of Nature.
  5. Ahmed MB (2012) Forest Genetic Resources Country Report Egypt. A thesis in Forests & Wood Technology Department, Faculty of Agriculture, University of Alexandria.
  6. Gad MM, Salem AM, El-Sayed, Hwida MF (2006) Clonal Propagation and Mass Production of Sequoia sempervirens, D. Don, Endl. Through tissue culture techniques. Proceedings of First International Conference on Strategy of Botanic Garden, Bulletin of CAIM- Herbarium 7:53-69.
  7. Egyptian Pharmacopoeia (2005) General Organization for Governmental Printing Affairs, Cairo, Egypt.
  8. Adams RP (2009) Identification of Essential Oils components by Gas Chromatography/Mass spectroscopy. Allured publishing Corporation, Carol Stream, Illinois, USA.
  9. Burits M, Bucar F (2000) Antioxidant activity of Nigella sativa essential oil. Phytother Res 14: 323-328.
  10. Bauer AW, Kirby WM, Sherris JC, Turck M (1966) Antibiotic susceptibility testing by a standardized single disk method. Am J ClinPathol 45: 493-496.
  11. Farraro MJ, Craig WA, Dudley MN, Eliopoulos GM, Hecht DW, et al.(2000) Performance Standards for Antimicrobial Disk Susceptibility Tests Approved Standard M2-A7, 7thEdn, National Committee for Clinical Laboratory Standards: Wayne, PA, USA,pp: 1-26.
  12. Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, et al. (1990) New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst 82: 1107-1112.
  13. Sefidkon F, Meshkizadeh S, Shahrzad SH (2002) Comparison Between Oil Composition of Sequoia Sempervirens From Tissue Culture And Main Sample.Journal of Chemical Ecology 17: 1837-1857.
  14. Francisco JE, Jean HL(1991) Effect of some leaf essential oil phenotypes from coastal redwood on growth of its predominant endophyticfungus, Pleuroplaconema sp. Journal of Chemical Ecology 17: 1837-1857.
  15. Nirmal SA, Chavan MJ, Tambe VD, Jadhav RS, Ghogare PB(2005) Chemical composition and antimicrobial activity of essential oil of Wedelia trilobata leaves. Indian J Nat Prod 21:33-35.
  16. Koheil MA (2000) Study of the essential oil of the flower-heads of Wedelia trilobata (L.) Hitch. J Pharm Sci 26:288-293.
  17. Craveiro AA, Matos FJA, Alencar JW, Machado MIL, Krush A, et al. (1993) Volatile constituents of two Wedelia species. J Essent Oil Res 5:439-441.
  18. Bajpai VK, Al-Reza SM, Choi UK, Lee JH, Kang SC (2009) Chemical composition, antibacterial and antioxidant activities of leaf essential oil and extracts of Metasequioaglyptostroboides Miki ex Hu. Food Chem Toxicol 47: 1876-1883.
  19. MarĂ³stica MR, Silva TE, Franchi GC, Nowill A, Pastore GM, et al.(2009) Antioxidant potential of aroma compounds obtained by limonene biotransformation of orange essential oil. Food Chem; 116: 8-12.
  20. Wang W, Wu N, Zu YG, Fu YJ (2008) Antioxidative activity of Rosmarinus officinalis L. essential oil compared to its main components. Food Chem 108: 1019-1022.
  21. Hirose M, Ozaki K, Takaba K, Fukushima S, Shirai T, et al.(1991) Modifying effects of the naturally occurring antioxidants gamma-oryzanol, phytic acid, tannic acid and n-tritriacontane-16, 18-dione in a rat wide-spectrum organ carcinogenesis model. Carcinogenesis 12: 1917-1921.
  22. Hwang PL (1992) Inhibitors of protein and RNA synthesis block the cytotoxic effects of oxygenated sterols. Biochim Biophys Acta 1136: 5-11.
  23. Hahismoto SS, Yazawa T, Asao A, Faried T, Nishimura K, et al.(2008) Novel sugar-cholestanols as anticancer agents against peritoneal dissemination of tumor cells. Glycoconjugate Journal 25: 531-544.
  24. Legault J, Dahl W, Debiton E, Pichette A, Madelmont JC (2003) Antitumor activity of balsam fir oil: production of reactive oxygen species induced by alpha-humulene as possible mechanism of action. Planta Med 69: 402-407.
  25. Sylvestre M, Legault J, Dufour D, Pichette A (2005) Chemical composition and anticancer activity of leaf essential oil of Myrica gale L. Phytomedicine 12: 299-304.
  26. Sylvestre M, Pichette A, Longtin A, Nagau F, Legault J (2006) Essential oil analysis and anticancer activity of leaf essential oil of Croton flavens L. from Guadeloupe. J Ethnopharmacol 103: 99-102.
  27. Tatman D, Mo H (2002) Volatile isoprenoid constituents of fruits, vegetables and herbs cumulatively suppress the proliferation of murine B16 melanoma and human HL-60 leukemia cells. Lett 175: 129-139.
  28. Hou J, Sun T, Hu J, Chen S, Cai X, et al.(2006) Chemical composition, cytotoxic and antioxidant activity of the leaf essential oil of Photiniaserrulata. Food Chem. 103:355-358.
  29. Boulos L (1993) In medicinal plants of North Africa. 218 St. Clair River Drive, Box 344, Algonac, Michigan 48001: Reference publications, Inc., p: 286.
  30. Farjon A (1998) World Checklist and Bibliography of Conifers. Royal Botanic Gardens, Kew,p: 300.
Citation: Taha KF, Shakour ZTA (2016) Chemical Composition and Antibacterial Activity of Volatile Oil of Sequoia sempervirens (Lamb.) Grown in Egypt. Med Aromat Plants 5:245.

Copyright: © 2016 Taha KF, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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