Journal of Medical Diagnostic Methods
Open Access

Diagnosis Process for Blood Analysis

Memmeico Jussica^{* *}Correspondence: Memmeico Jussica, Department of Diagnostic, University of Benin, Benin, Nigeria, Email:

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About the Study

High-resolution dark field microscopy is used in Live Blood Analysis (LBA), also known as live cell study, or nutritional blood analysis, to study living blood cells. Some advocates of alternative medical advocate claims that live blood analysis is capable of making a variety of diagnoses. In order to develop accurate and analytical diagnosing applications, the area of medical diagnostics has worked to investigate specific species of enzymes. The red blood cells are examined using Raman hyper spectroscopy to detect Celiac Disease (CD). A prevalent autoimmune condition called CD, which affects 1% of people, is common. An individual with CD will experience a powerful immunological reaction after consuming gluten, which will seriously harm their small intestine. Significant long-term health consequences that vary in intensity may develop if the condition is undetected. Therefore, it is essential to detect the sickness as soon as possible in order to stop the development of new issues.

However, existing approaches of CD detection are costly, intrusive, and time-consuming, the objective of this work was to provide a non-invasive, low-cost, reliable, and conclusive approach for diagnosing CD. Individual red blood cells from individuals with CD and also from healthy controls who avoid gluten were examined using Raman hyper spectroscopy. To assess the gathered Raman spectrum data for medical diagnostics, Partial Least Squares Discriminant Analysis (PLS-DA) was applied. The PLS-DA predictions algorithm's performance was assessed using ROC curve analysis, which produced a 100% effective external validation of the created approach at the donor level.

It is proven that Raman hyper spectroscopy in conjunction with chemo metric analysis may successfully assess red blood cells for the precise detection of CD in a non-invasive, straightforward, and affordable method. An effective non-invasive and label-free approach for active Tb diagnosis has been developed using auto fluorescence spectroscopy in the combination with a multivariate analytic technique for blood plasma analysis. The initial and foremost derivative fluorescence spectrum of blood plasma samples from 12 diagnosed active pulmonary TB patients and 9 healthy (control) volunteers are recorded, and a clear intensity difference between the two groups is detected.

To minimize dimensionality and emphasise spectral differences, the Principal Component Analysis (PCA) technique is performed to fluorescence spectra. The two cutting-edge analytical techniques for assessing blood cells (white blood cells, red blood cells, and thrombocytes, or TC) in Striped bass Morone saxatilis (Walbaum, 1792).

Blood samples from 20 adult M. saxatilis specimens raised in a Recirculating Aquaculture System (RAS) at a fish farm in Acate, Spain, were analysed for this purpose using the automatic blood cell count and flow cytometry analysis in Italy. A fluorescentisoindole prototype is developed by reacting HCN with taurine, naphthalene dialdehyde, and other chemicals in a capture solution using high-speed headspace transfer. With just 25 L of blood, a straightforward spectrofluorometric assay of the product may quantitatively analyse CN from whole blood in 60 seconds Complete Blood Count (CBC) inflammatory variables are linked to a decline in the success rate of in vitro embryo production (IVP) in females. It is still unclear how blood indices and In Vitro Fertilisation (IVF) results in cattle relate to one another. Oocytes were removed from heifers (n=60) using Ovum Pick-up (OPU) and individually implanted with sperm.

Linear Discriminant Analysis (LDA) method on the two groups' blood plasma to categories it, demonstrates that the PCA-LDA diagnostic model can accurately diagnose patients with TB in 95.2 percent of the time, with 91.7% sensitivity and 100% specificity. Utilizing the leave-one-out cross validation approach, the diagnostic quality was evaluated and verified.

This is the first instance where blood plasma auto fluorescence spectroscopy has been shown to be a promising technique for identifying and screening pulmonary in TB illness.