Journal of Cell Science & Therapy

Journal of Cell Science & Therapy
Open Access

ISSN: 2157-7013

+44 1300 500008

Image - (2014) Volume 5, Issue 5

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Impaired Cardiac Function in Cardiac-Specific SIRT1-Deficient Mice

Feng Cao*
Chief Physician, Cardiology Deptartment, Chinese PLA General Hospital, Beijing, P.R. China
*Corresponding Author: Feng Cao, Associate Director, Chief Physician, Cardiology Deptartment, Chinese PLA General Hospital, 28# Fuxing Street, Beijing, 100853, P.R. China, Tel: 86-10-55499449 Email:

Abstract

Cardiac specific SIRT1 knockout mice were generated through the Cre/loxP system. Briefly, loxP-SIRT1 mice and Myh6-Cre mice were crossed to get SIRT1+/- mice.

Description

Cardiac specific SIRT1 knockout mice were generated through the Cre/loxP system. Briefly, loxP-SIRT1 mice and Myh6-Cre mice were crossed to get SIRT1+/- mice. Then, SIRT1+/- female mice were mated with SIRT1+/- adult males to generated SIRT1-/- mice. RT-PCR revealed the gene type of SIRT1+/- and SIRT1-/- mice (A).

The low expressions of SIRT1 in SIRT1+/- and SIRT1-/- mice were confirmed by Western Blot (B). The heart/body weight was increased only in SIRT1-/- mice.

Echocardiography results (D) showed that cardiac function was impaired in SIRT1-deficient mice, with the decreased ejection fraction (EF) in SIRT1-/- mice (E).

Equation

Citation: Feng Cao (2014) Impaired Cardiac Function in Cardiac-Specific SIRT1-Deficient Mice. Cao, J Cell Sci Ther 2014 5:I101.

Copyright: © 2014 Cao F. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use,distribution, and reproduction in any medium, provided the original author and source are credited.
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