Journal of Clinical Chemistry and Laboratory Medicine
Open Access

Importance of Laboratory Techniques

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Introduction

Hands-on encounters fundamentally advance learning at all degrees of science training when fittingly structured and guided by qualified teachers. During hands-on science exercises, understudies legitimately and securely examine synthetic properties and responses, using research center mechanical assembly and instruments. These exercises are basic for learning science and improving science proficiency. Electronic and PC reenacted exercises may help increment understudy presentation to science, diminish costs, and dispense with risky waste and wellbeing concerns; be that as it may, these apparatuses can't be considered as comparable substitutions for hands-on lab encounters.

The Society accepts that there is no equal substitute for hands-on exercises where materials and gear are utilized securely and understudy encounters are guided. The Society bolsters continued speculations to give the offices, hardware, educational programs, and expert advancement required for successful hands-on research center science encounters from kindergarten through post-optional instruction [1].

Hands-on experiences in a general sense advance learning at all degrees of science preparing when fittingly organized and guided by qualified instructors. During hands-on science works out, understudies really and safely look at manufactured properties and reactions, utilizing research focus mechanical get together and instruments. These activities are essential for learning science and improving science capability. Electronic and PC reenacted activities may assist increase with understudying introduction to science, lessen costs, and shed hazardous waste and prosperity concerns; in any case, these mechanical assemblies can't be considered as practically identical replacements for hands-on lab experiences.

The Society acknowledges that there is no equivalent substitute for hands-on practices where materials and apparatus are used safely and understudy experiences are guided. The Society supports proceeded with theories to give the workplaces, equipment, instructive projects, and master headway required for fruitful hands-on research focus science experiences from kindergarten through post-discretionary guidance [2].

Blotting Techniques

Smudging is the most well-known lab strategy generally utilized in the field of cell and sub-atomic science. It is an amazingly helpful multi-step procedure utilized in the recognizable proof of various biomolecules, for example, DNA, mRNA, and protein during various phases of quality articulation or for distinguishing explicit proteins from a mind boggling blend of proteins after extraction from cell – the applications are many [3].

Techniques for Extraction & Storage of Biomolecules

The most widely recognized biomolecules utilized in research center analysis incorporate DNA, RNA, and Proteins. There are fixed conventions for extraction of these biomolecules. RNA being exceptionally touchy to debasement must be removed with the most extreme consideration. The extraction convention incorporates the utilization of various reagents with various jobs playing in the extraction of the most perfect biomolecule. It is significant for the analyst to get familiar with the essentialness of every reagent and use them appropriately according to the necessity of the examination. It is additionally required to have an unmistakable thought with respect to legitimate capacity state of the atoms to store them for long haul use [4].

Gel Electrophoresis

For any sub-atomic investigation, for example, dissecting DNA, RNA and protein must be finished by gel electrophoresis. So it is essential to pick up utilizing the electrophoretic device and furthermore to cast the gel appropriately. The nature of gel will give a straightforward and precise outcome. Top 10 Lab Techniques Every Researcher Must Know While getting ready gel for throwing the most significant part is a precise extent of fixing (agarose%/PA), as this will influence the pore size which thus will decide the exploratory result. While agarose gel is utilized for the examination of DNA, PAGE (polyacrylamide gel electrophoresis) and SDS (Sodium Dodecyl Sulfate)- PAGE are utilized for proteins. Agarose gel electrophoresis is the best way DNA examination dependent on size. Gel electrophoresis is broadly utilized in atomic science and organic chemistry labs in territories, for example, criminological science, preservation science, and medication [5].

Conclusion

The laboratory techniques are very important in a researcher’s life. These skills play a significant role in order to perform various experiments and to run various tests. Without proper knowledge, one can not apply these techniques in projects and researches. These techniques are vital for most of the experiments.

References

1. Van der Vleuten CP. “Competency-based education is beneficial for professional development,” Perspectives on Medical Education.2015;4:323-325.
2. Goodyear N, Kim S, Reeves M, Astion ML. “A 2-year study of Gram stain competency assessment in 40 clinical laboratories,”. American Journal of Clinical Pathology.2006;125:28-33.
3. Wiebusch FB, Wittermann JK, Saroff SA. “Report of a competency based education model in continuing education,”. Virginia Dental Journal.1976;53:15-26.
4. Rego SL, Zakhem E, Orlando G, Bitar KN. “Bioengineered human pyloric sphincters using autologous smooth muscle and neural progenitor cells,”. Tissue Engineering Part A.2016;22:151-160.
5. Atala A, Murphy S. “Regenerative medicine,”. The Journal of the American Medical Association.2015;313:1413-1414.