In latest times, genome sequences of different organisms are available in numerous databases by applying next generation sequencing technology to collect information to a larger quantity. As a result, increasing quantities of records approximately hypothetical proteins deposited in sequence databases instead of experimentally isolated facts in Protein data bank (PDB). Hypothetical proteins are generally fore casted to be expressed from an open reading frame (ORF). Those proteins don't have any experimental proof concerning their functions. At gift, it's far assumed that 50% protein of a genome is hypothetical proteins. This encourages in silico take a look at of a hypothetical protein utilizing experimental information [1]. Valid structural and functional annotations of HPs of unique genome may also spark off the finding of latest structures and also new functions and help to offer extra protein pathways and cascades, in this way finishing our rough records on the variety of proteins [2]. The fully sequenced genomes of numerous organisms offer huge quantities of facts approximately cellular biology (see the genomes indexed at the internet website of The Institute for Genome studies: www.tigr.org). It’s far an imperative venture of bioinformatics to apply this fact in coming across the function of proteins. Functional assignments of genes come primarily from biochemical experimentation, which may be prolonged through matching recently sequenced proteins to people who have already been characterized [3].

The common bean is the most significant food bank for direct human consumption in the world. The crop is a main creation in worldwide commerce and is created and consumed by huge numbers of the rural and town unfortunate in Latin America and Africa [4]. Common bean (Phaseolus vulgaris) is the major green bean for human digestion and has a role in maintaining agriculture outstanding to his capacity to fix atmosphere nitrogen (N2). We accumulated 473 MB of the 587-Mb genome and hereditarily anchored 98% of this sequence in 11 chromosome-scale pseudo molecules [5]. However, in unkindness of the significance of this yield species, its genetics have remained poorly characterized. For illustration, only a limited morphological and seed and flower color markers have remained used to grow a rudimentary linkage map. More lately, a few isozyme and protein markers take remained additional to the map. Although cytogenetic analysis takes to remain troubled by the minor size of the chromosomes, five primary trisomics take remained characterized [6].

In current centuries, numerous hypothetical proteins have been created in the genome of several life systems. In some circumstance, because of a few limitations, for illustration, the expenditure and time required for examining methodologies, whole genome annotations have not accomplished until now. In adding, the wide amount of theoretical proteins in a genome creates their analysis a difficult task. Bioinformatics approaches using distinguishing algorithms and data-bases to evaluation protein capacity would be a decent altered option for laboratory exploration ability based procedures. As these algorithms and databases are in graceful of experimental results analysis, they can be a convincing aims to complete functionality and structural annotation of hy pothetical proteins [7].

In the present study, the Common bean (Phaseolus vulgaris) hypothetical protein>XP_007152511.1, belongs to AAA group was selected as the primary amino acid sequence of the protein is available however structural details are not available. This study meant to examine the physiochemical and secondary structure mechanisms, to produce the first three-dimensional (3D) model completed by Ab initio method, and finally to complete functional an notations. The result of this work resolve suitable for better comprehension of the tail of this protein and determining other novel proteins and their functions by the same way that we have accomplished for Common bean (Phaseolus vulgaris) protein.

Sequence retrieval

The amino acid sequence of the common bean (Phaseolus vulgaris) hypothetical protein>XP_007152511.1 was retrieved from the Uniprot database (www.uniprot.org).

Physiochemical analysis of the protein

Examination of the physiochemical properties of the studied protein such as molecular weight, theoretical pI, amino acid composition, atomic composition, instability index, and grand average of hydrophobicity (GRAVY) remained to complete using ProtParam tool (http://web.expasy.org/protparam/) [8].

Secondary structure analysis

The server SOPMA was applied for secondary structure calculations (helix, sheets, and coils) of the hypothetical protein [9]. In adding to that, the PSIPRED [10] and Predict Protein [11] servers were also exploited to confirm the results achieved from SOPMA.

Subcellular localization prediction

Subcellular localization of common bean (Phaseolus vulgaris) was predicted by PSORT [12]. Results remained also cross-checked through subcellular localization predictions acquired from SOSUI server and Predict-Protein servers [13].

Homology modeling of the hypothetical protein

The possible 3D structure of the protein>XP_007152511.1 was created over alignment approach in protein structure homology modeling server SWISS-MODEL [2,14] with the full amino acid sequence of the protein in FASTA format.

Quality assessment of the 3D model and visualization

The early structural model achieved, was checked for acknowledgement of mistakes in 3D structure [11] by ERRAT and Verify3D programs involved in structural examination and confirmation server SAVES (http://nihserver.mbi.ucla.edu/SAVES/) [15,16]. The Ramachandran plots for the model remained built using the RAMPAGE server [17], viewing the percentage of protein residues in the favored, allowed and outlier sections. The visualization of creating model was accomplished by Discovery studio 4.1 [14].

Functional annotation of the protein

Common bean (Phaseolus vulgaris) hypothetical protein>XP_007152511.1 was examined for the function. Three different bioinformatics tools and databases including ProtFun 2.2 [15], ProFunc [18], and NCBI Conserved Domains Database (NCBICDD) [19] remained utilized for this goal. More over, KEGG automatic annotation server (KAAS) was used to examine the association of (Phaseolus vulgaris) hypothetical protein in the metabolic pathways [14].

Submission of the model in protein model database

The model created for Common bean (Phaseolus vulgaris) hypothetical protein>XP_007152511.1 was successfully submitted in Protein Model Database (PMDB) (http://bioinformatics.cineca.it/PMDB/).

Physiochemical characteristics of XP_007152511.1

The ExPASy’s ProtParam server was developed to study the theoretical physiochemical characteristics of the amino acid sequence of hypothetical protein XP_007152511.1. The enor mous majority of the calculations in this server show protein steadiness and stability, in light of the detail that the stability is recognized with its suitable function ability [3]. The protein was predicted to be contained 220 amino acids, with a molecular weight of 24330.23 Daltons and an isoelectric point (PI) of 6.38 demonstrated a negatively charged protein. The instability index of the protein was computed to be 47.86, which demonstrated this protein as stable.

The GRAVY index of -1.025 is expressive of a hydrophobicity and solubility of protein. The most plenteous amino acid residue was observed to be Alanine (20), followed by Leucine (19) and the most minimal amino acid as Cytosine and tryptophan (1). The sequence had 40 nega tively charged residues (Aspartic acid+Glutamic acid) and 39 positively charged residues (Arginine+Lysine). The molecular formula of the protein remained found as C¹⁰⁵⁰ H¹⁷⁰¹ N³¹¹ O³⁴³ S⁵.

Subcellular localization of XP_007152511.1

Protein subcellular localization predictions contain the computational probability of somewhere protein survives in a cell. Predicting subcellular localization of unidentified proteins can provide information around their cellular functions. This information might be applied in understanding disease mechanism and developing drugs [20]. The subcellular localization of the query protein was anticipated to be a nuclear protein, analyzed by SOSUI and confirmed by PSORTb v3.2.0 and Predict Protein severs.

Secondary structure of XP_007152511.1

First the secondary structure of the protein remained predicted by SOPMA server. The alpha helix was found to be the most predominant (36%), followed by random coil (47%) and extended strand (8%). Likewise, beta turn was found as 7.73%. Sec ond, the similar results stayed found from Predict-Protein and PSIPRED servers. The representative secondary structure of XP_007152511.1 obtained from the PSIPRED server is shown in Figure 1.

Figure 1: Predicted secondary structure of the Common bean (Phaseolus vulgaris) hypothetical protein >XP_007152511.1 by PSIPRED server.

Homology modeling of XP_007152511.1

We assume these uncharacterized proteins infinite unexplored field with numerous occasions, both as medicinal and industrial tools. In Silico analysis may help with determining the biological functions of such un-characterized proteins. This can be encouraged by anticipating the 3D structure of the target protein. At the point when the uncertainly experimented structure is isolated, similar or homol ogy modeling can now and then give a helpful 3D model to the protein of attention that is identified with no less than one known protein structure. Homology modeling predicts the 3D structure of an assumed protein sequence build principally with re spect to its alignments to one or more proteins of known structure [21]. To perform the homology modeling, the query sequence was given as input in SWISS-MODEL server. The server consequently performed BLASTP search for each protein sequence to identify templates for homology modeling. The highest tem plate identity was 20% which showed that XP_007152511.1 hypothetical protein is novel and no similar template structure is present in any databases. We predicted the 3D structure of XP_007152511.1 hypothetical protein by Ab initio method through Phyre2 server and 3D Jigsaw server which gave 99.3% con fidence in model. The 3D model was viewed by Discovery studio 4.1 and shown in Figure 2.

Figure 2: Structural analysis of Common bean (Phaseolus vulgaris) hypothetical protein >XP_007152511.1.

Quality assessment and visualization

Reliability of the created model was initially checked by ERRAT that analyzed the statistics of non-bonded interactions between diverse atom types, based on characteristic atomic interactions. The overall quality factor was found as 26.88%, sufficient enough to use this model. As demonstrated by the Verify3D program, the results showed that 51.08% of residues had an average 3D (atomic model) – 1D (amino acid) score ≥0.2 meaning that this structures was compatible and genuinely good.

Ramachandran plots were resolved. Z-score of the query model was checked from PROSAweb. The model’s Z-score was not shown due to novelty of XP_007152511.1 protein. The Z-score is used to estimation the quality of model using structured solved proteins as reference [22]. The stereo chemical quality of the model protein was examined using Ramachandran plots through the RAMPAGE server. Ramachandran plot analysis observed 81.2% of residues of the protein’s model structure in the favored region, with 11.0% and 7.8% residues in allowed and outlier regions, individually, indicating that the model was reliable and of good quality shown in Figure 3. The final protein structure was deposited in PMDB and is available under ID PM0080295.

Figure 3: Ramachandran plot for the 3D model of the studied hypothetical protein XP_007152511.1 by RAMPAGE server.

Functional annotation of XP_007152511.1

We utilized three web tools to search the potential functions of XP_007152511.1. In light of predictions made by Prot Fun 2.2, and KEGG (KAAS), XP_007152511.1 was sug gested as a translation and also acts as a non-enzyme. It helps in Trans membrane bar riers, protein protein binding, metabolic process, cellular process. The protein was found to be central for the translation process, involved in signaling and cellular processes, transfrase activity, and catalytic activity.

Comparative genome analysis of XP_007152511.1

We utilized NCBI Blast search tool for comparative genome analysis of XP_007152511.1 hypothetical protein of Phaseolus vulgaris with other plant ge nomes. In the result the XP_007152511.1 showed highest similarities with other uncharacterized hypothetical proteins of several plants [23,24].

The present study was directed to create the first 3D structure and propose possible functions of the Phaseolus vulgaris hypothetical protein XP_007152511.1. The 3D model of the protein was constructed using Ab initio method as well as refined by few structural assessment methods and the final outcome was genuinely great. We observed that this novel protein is a stable nuclear protein and function as a translation factor process, non-enzyme act. The protein was observed to be cen tral for the translation process, also involved in, trans membrane barriers, protein protein interaction, metabolic process, cellular process, transferase activity, catalytic activity processes and signaling and cellular process. From genomic simi larities we conclude that this hypothetical protein may be checked for same function as of XP_007152511.1. Moreover, this sort of methodology could be help ful in the structure and functions prediction of other uncharacterized proteins.