Medicinal & Aromatic Plants

Medicinal & Aromatic Plants
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ISSN: 2167-0412

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Mini Review - (2014) Volume 3, Issue 4

Pharmacological Properties and Chemical Constituents of Murraya paniculata (L.) Jack

Katayoun Sayar1, Mohammadjavad Paydar2* and Belinda Pingguan-Murphy1
1Department of Biomedical Enginnering, Faculty of Engineering, University of Malaya, Kuala Lumpur, 50603, Malaysia
2Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Malaysia
*Corresponding Author: Mohammadjavad Paydar, Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Malaysia, Tel: +603 79674711 Email:

Abstract

Murraya paniculata (L.) Jack belongs to the family Rutaceae and is mostly distributed throughout South Asia
to Australia. Many pharmacological effects of the plant have been reported, and range from antinociceptive,
antioxidant, anti-diabetic, antimicrobial to analgesic activities. A wide range of different compounds consisting of coumarins, alkaloids, phenols, terpenoids and flavonoids have been identified from different parts of the plant and have been evaluated for various biological activities. The aim of this review is to cover the biological activities and the active compounds derived from M. paniculata to provide more insights and spur further investigations that would lead to production of more effective and economical alternative medicine from the plant.

Keywords: Murraya paniculata, Chemical constituents, Biological activities

Introduction

Murraya is a genus of flowering plants, closely related to citrus. It is in the subtribe Clauseninae, which are known technically as the remote citroid fruit trees. Murraya paniculata (L.) Jack, commonly known as Orange Jessamine, is a tropical, evergreen plant with tiny, white, scented flowers, which is cultivated as an ornamental tree or hedge (Figure 1). It belongs to the family Rutaceae and can be commonly found in South Asia and Australia. Various parts of this plant have been used in traditional medicine. In BangladeshM. paniculata leaves extract is orally used to alleviate pain [1]. In the Philippines, leaves were also used to treat diarrhea and dysentery because of their stimulant and astringent activities [2]. In India, people sometimes used root bark ofM. paniculata as remedy for coughs, hysteria and rheumatism [3]. Furthermore, cooked leaves and boiled twigs applied to assuage inflamed joints and stomachache respectively in India [4].

medicinal-aromatic-plants-morphology-Murraya-paniculata

Figure 1: The morphology of Murraya paniculata (L.) Jack.

There are many reports on pharmacological effects of the plant including antinociceptive [1,5], antioxidant [6,7] and anti-diabetic [4], to antimicrobial [4] and analgesic activities [8]. Several research groups have reported isolation of effective substances like alkaloids [9], phenols [4], terpenoids [10] and flavonoids [4,11-13] from leaves, fruits, flowers and root barks ofM. paniculata as health remedy. In view of the importance of M. paniculata, we describe its antinociceptive, antioxidant, anti-diabetic, antimicrobial and analgesic properties.

Chemical Constituents of Murraya paniculata (L.) Jack

M. paniculata has been investigated for its bioactive compounds by many research groups. To date, various compounds were identified, ranging from indole alkaloids, coumarins, phenols, terpenoids to flavonoids. Besides, 60 compounds have been identified from volatile and essential oil extracted fromM. paniculata leaves. The chemical components from different parts ofM. paniculata were identified using chromatographic techniques and the structures were elucidated using spectroscopic techniques. A number of these compounds exhibited significant biological activities, which serve as the scientific evidence for the traditional usage of M. paniculata.

In 1986, an anti-implantation alkaloid, called Yuehcukene, 1β-(3,- indolyl-7,9α,9β-trimethyl-5β,8,9,10β-tetrahydroindano-[2,3-b] indole was also isolated fromM. paniculata leaves [14]. Moreover, the two indole alkaloids, murrayacarine [15] and murrayaculatine [16] were isolated from root bark and flowers ofM. paniculata respectively.

In early 1980s, different research groups isolated 3’,4’,5,5’,7,8-hexamethoxyflavone (Figure 2a) and 3,3’, 4’,5,5’,7,8-heptamethoxyflavone (Figure 2b) from the methanolic extract ofM. paniculata leaves [17,18]. Later, a flavone named 3,5,7,3’,4’,5’- hexamethoxyflavone, was isolated fromM. paniculata flower [16]. Other research groups isolated eight flavonoids fromM. paniculata leaves [12,19] and ten flavonoids from the peel and pulp of the fruits of the plant [20].

medicinal-aromatic-plants-Chemical-structure-compounds

Figure 2: Chemical structure of major compounds identified in Murraya paniculata (L.) Jack.

Furthermore, three coumarins known as meranzin hydrate (Figure 2c), murpanidin (Figure 2d) and murragatin (Figure 2e) were isolated fromM. paniculata leaves extract [18]. Other coumarins including 3-formylindole,omphalocar-pin,5,7-dimethoxy-8-(3’-methyl-2’- oxobuty coumarin, coumurrayin, murragleinin, omphamurin, murraol, (-)- murracarpin, (±)-murracarpin, mupanidin, mexoticin, murrangatin, and ferulyl esters were also isolated from M. paniculata root bark [15]. While from the fresh flowers of this plant, yuehgesin-A, yuehgesin-B, yuehgesin-C, and 22 compounds were characterized [21]. Besides nine coumarins, omphamurrayone, murralongin, isomurralonginol isovalerate, murrangatin, minumicrolin (murpanidin), coumurrayin, toddalenone, auraptene and toddasin were also identified fromM. paniculata leaves acetone extract [22] and methyl 2,5-dihydroxycinnamate and murrayatin from methanolic extract [23].

As mentioned, 60 compounds have been aslo identified from volatile and essential oil extracted fromM. paniculata leaves. The major components were t-caryophyllene (Figure 2f), γ-elemene (Figure 2g), perolidol, β-elemene (Figure 2h), spathulenol (Figure 2i), caryophyllene oxide (Figure 2j), β-caryophyllene, germacrene D (Figure 2k) and 4-methylene-6-(1-propenylidene)-cyclooctene (Figure 2l) [10,24,25].

Antioxidant Activities

Reactive oxygen species (ROS) are natural products of oxygen metabolism in biological systems which comprise in energy production, defense mechanism against infection and phagocytosis [26,27]. However, catastrophic biological oxidation can be observed while the production of ROS is enhancing. When cells have been exposed to excess ROS, some of the oxidative conversions like DNA affliction, lipid peroxidation and enzyme inactivation cause detrimental modification in cell function [27-29].

In recent years the utilization of substances from natural sources with antioxidant properties have been boosted because the preservation toward multitudinous disease [30-32]. Some problems concerning the conservation and toxicity of synthetic antioxidants lead to contributing more investigations on natural antioxidants derived from plant sources. Strong potential of plant antioxidants make them a crucial area of research [30-34]. Some plant source antioxidants are beta-carotene, selenium (Se) and flavonoids including flavanols, flavanones, flavones, iso-flavones, catechins, anthocyanins and proanthocyanidins [27].

Zhang et al. (2011) reported the antioxidant property of M. paniculata for the first time [7]. They detected seventy polymethoxylated flavonoids (PMFs) in the leaves extract and thirty nine PMFs in the branches extract ofM. paniculata (Table 1) [35]. PMFs include a particular group of flavonoids responsible for numerous biological properties including antioxidant activity [36].

Identified PMFs in the leaves Identified PMFs in the branches
Dihydroxy-dimethoxyflavone Heptamethoxyflavone
Monohydroxy-trimethoxyflavone Trihydroxy-dimethoxyflavone glycoside
Trihydroxy-dimethoxyflavone Tetrahydroxy-dihydroxyflavone glycoside
Tetramethoxyflavone Monohydroxy-dimethoxyflavone glycoside
Dihydroxy-trimethoxyflavone Monohydroxy-trimethoxyflavone glycoside
Tetramethoxyflavanone
or
Tetramethoxychalcone
Heptamethoxyflavanone
or
Heptamethoxychalcone
Monohydroxy-trimethoxyflavone Monohydroxy-tetramethoxyflavone glycoside
Monohydroxy-tetramethoxyflavanone
or
Monohydroxy-tetramethoxychalcone
Monohydroxy-trimethoxyflavanone
or
Monohydroxy-trimethoxychalcone
Trihydroxy-trimethoxyflavone Monohydroxy-pentamethoxyflavone
Pentamethoxyflavone Monohydroxy-trimethoxyflavone
Pentamethoxyflavanone
or
Pentamethoxychalcone
Pentamethoxyflavanone
or
Pentamethoxychalcone
Dihydroxy-tetrahydroxyflavone Monohydroxy-trimethoxyflavone
Monohydroxy-pentamethoxyflavone Pentamethoxyflavone
Monohydroxy-pentamethoxy
or
Monohydroxy-pentamethoxychalcone
Hexamethoxyflavanone
or
Hexamethoxychalcone
Trihydroxy-tetramethoxyflavone Tetramethoxyflavone
Hexamethoxyflavone Hexamethoxyflavonem
Hexamethoxychalcone Dihydroxy-dimethoxyflavone
Monohydroxy-hexamethoxyflavanone
or
Monohydroxy-hexamethoxychalcone
Tetramethoxyflavanone
or
Tetramethoxychalcone
Monohydroxy-hexamethoxyflavone Heptamethoxyflavone

Table 1: Polymethoxylated flavonoids (PMFs) detected in Murraya paniculata (L.) Jack.

In 2005, Rohman and Sugeng reported significant antioxidant activity of the ethanol extract ofM. paniculata leaves in linoleicthiocyanate and 2,2-diphenyl-l-picryl hydrazyl (DPPH) methods [6]. They showed that the IC50 ofM. paniculata leaves extract is 126.17μg/ mL whereas, the IC50 of vitamin E the positive control is 8.27μg/mL. Furthermore, Chen et al. (2009) found that the 500μg/mL acetone extract ofM. paniculata leaves displayed 72% inhibitory effect towards tyrosinase activity [37]. Besides, the 100μg/mL extract was able to inhibit 62% of lipoxygenase (LOX) and 10% of xanthine oxidase (XO) activities.

In 2011, Shaikh et al. isolated the secondary metabolite 2’-O-ethylmurrangatin from the leaves ofM. paniculata by using spectroscopic techniques [38]. They found its significant activity towards lipoxygenase. 2’-O-ethylmurrangatin displayed the IC50 of 28.1 (mM) which was more than the IC50 of baicalein the positive control 22.7 (mM). In 2012, Gautam et al. reported that antioxidants significantly increased in Sprague-Dawley rats after 14 days oral administration of ethanol extract ofM. paniculata leaves [4]. They found administration of 100, 200 and 400mg/kg ofM. paniculata leaves extract increased superoxide dismutase (SOD) from 80.43 to 109.31 U/mg protein, catalase (CAT) from 36.17 to 59.18 U/mg protein and glutathione peroxidase (GPx) from 1.51 to 2.12 U/mg protein. They mentioned antioxidant activity ofM. paniculata leaves extract is due to presence of alkaloids, flavonoids and phenolic compounds [4].

The results obtained in these in vitro and in vivo studies clearly demonstrate the high potential ofM. paniculata as a source of natural antioxidants. Further investigations are still required to confirm the antioxidant activity of the compounds and also to go through detailed mechanism of their activity.

Anti-diabetic Activities

Diabetes mellitus (DM) is a metabolic disorder caused by direct or indirect insulin inadequacy. Stimulation of glucose uptake into muscles and adipocytes determined by blood glucose level is under insulin control [39,40]. The glucose transporter Glut 4 can transport glucose from intra cellular pool to the plasma membrane in muscles and adipose tissues [41]. The glucose uptake can be measured by a fluorescent analogue of D-glucose, 2-[N-(7-Nitrobenz-2-oxa-1,3- diazol-4-yl)amino]-2-deoxy-D-glucose (2-NBDG) [42,43]. Another vital mechanism which assures glucose hemostasis is insulin secretion from pancreatic β-cells [44]. Inordinate insulin secretion may cause life menacing hypoglycaemia hence, deficient secretion influence perceptive or chronic damaging lead to DM [45]. Furthermore, hypoglycemic agents like alpha glycosidase inhibitors and sulphonylure as found in some medicinal plants extracts are considerable compounds for the treatment of DM type 2 by stimulating insulin secretion [46-48].

In 2012 Gautam et al. reported that oral administration of M. paniculata leaves extract included hypoglycemic agents such as sulfonylures significantly declined the glucose level in diabetic Sprague- Dawley rats. They showed 400 mg/kg ofM. paniculata leaves extract significantly reduced the glucose level (62.52 mg/mL) in diabetic rats compared to normal control group (94.78 mg/mL). In addition, they mentionedM. paniculata leaves extract can augment β-cell structure, cell membrane and nucleus in pancreas. Hypoglycemic action can be potentiating the insulin by enhancing the pancreatic secretion of it from β-cells of Langerhans islets or emancipating insulin from the bound form. Other studies showed that flavonoids in theM. paniculata leaves extract such as 5,7,3’,4’-tetramethoxy-flavone, 5,7,3’,4’,5’-pentamethoxyflavone, 5,6,7,3’,4’-pentamethoxy-flavone, 5,6,7,3’,4’,5’-hexamethoxy -flavone, and 7-hydroxy-5,3’, 4’-trimethoxy-flavone [49]. In 2009, Yongri et al. reported that the flavonoids inM. paniculata leaves extract evidently diminished the blood sugar level in ICR mice by increasing the insulin content and ameliorating the islet β-cells secretion index. However, the insulin resistance index significantly decreased [50]. Taken together, these in vivo results clarify thatM. paniculata leaves extract could have eminent therapeutic effect on the Diabetes Mellitus (DM).

Antimicrobial Activity

Various antimicrobial agents, either synthtic or natural, are employed against pathogenic microbes to reduce the risks of common infections [51]. The repeated or continued use of antibiotics had led to widespread antimicrobial resistance [52]. On one hand there are serious infections that need to be cured using antibiotics and on the other hand the side effects of available commercial drugs, highlight the urgent demand for investigation on antimicrobial activities of natural antimicrobial compounds.

Plant extracts have shown to be a potential source of the novel antimicrobial agents [53]. However, as Plants produce bioactive compounds for their defense mechanisms which can be toxic in nature [54], not only the antimicrobial properties of the plant extract but possible toxicity should also be considered for their safety/safe use.

M. paniculata extract has been traditionally used as an antimicrobial medication and is believed to demonstrate significant antimicrobial activities [4,55]. The leaves extract has been reported to be safe in its oral effective dose as it did not indicate toxicity when tested on rodents [4]. According to the research conducted on 50% ethanolic leaves extract of M. paniculata, acute oral administration of M. paniculata extract (2000 mg kg-1, single dose) did not cause any mortality, CNS and ANS toxicities in rats.

The extract ofM. paniculata leaves revealed the presence of alkaloids, flovonoids, phenolic compounds, which are all reported to have growth inhibition against gram positive and gram negative bacteria [4].

One of the mechanisms of phenolic compounds, that are known to possess antimicrobial activity, is by causing the leakage of cytoplasmic constituents such as protein, glutamate or potassium and phosphate from bacteria, which may occur as the result of disruption of cell peptidoglycan or damage of the cell membrane [56]. Flavonoids which are classified under phenolic groups have also demonstrated antimicrobial activity by inhibition of nucleic acid synthesis, cytoplasmic membrane function, and energy metabolism [57].

According to the research by Gautam et al., total phenolic and flavonoid content of different leaves extract ofM. paniculata including petroleumether extract, methanolic extract, ethanolic extract and hydroalcoholic extract were studied for their antibacterial activities [4]. At a concentration of 200mg/mL the inhibition zone of ethanolic extract and hydro-alcoholic extract tested on several human pathogenic bacteria, including E. coil, K. pneumoniae, S. typhi, E. faecalis, P. aeruginosa, S. flexinerrii, S. aureus and S. sonneii showed mild to moderate activity of 8-11mm. Petroleumether extracts indicated 8-12 mm of inhibition zone and the methanolic extract had highest antibacterial activity of 9-14 mm among other compound [4].

Analgesic and Antinociceptive Activity

Scientific exploration of new pain relieving herbal drugs with minimum side effects are in high demand [58]. Oral administration of M. paniculata leaves extract was used in traditional medicine in many places of Bangladesh for abatement of pain [1,59]. According to the study conducted by Podder et al., analgesic activity of M. paniculata bark extract has been practically proven [8]. They applied a method to indicate antinociceptive activity by testing the inhibitory ability of the sample against acetic acid induced writhing [60]. In their investigation, M. Panuculata bark extract was administered to mice at an oral dose of 200 and 400 mg/kg body weight. At the given doses, the extract indicated 37 (p<0.001) and 45% (p<0.001) inhibition of writhing respectively by reducing the frequency of acetic acid. Besides, the 19% (p<0.05) elongation of flicking time after 120 min was also observed [8]. This study concurred with the previous investigation by Chevallier (1996), indicating significant analgesic effect ofM. paniculata bark extract in albino mice [61].

In 2009, Sharker et al. used a similar method to measure the antinociceptive activity ofM. paniculata leaves ethanol extract [1]. They injected the 0.7% of acetic acid solution to the Swiss albino mice and then oral administration of 250 and 500 mg/kg of M. paniculata leaves extract produced significant antinociceptive activity of 26.27 and 66.67 writhing inhibitory percentage in mice in a dose dependent manner. In addition, Narkhede et al. reported oral administration of ethanol extract ofM. paniculata leaves at the doses of 50, 100 and 200 mg/kg significantly inhibited the writhing at the rate of 28.84%, 54.93% and 67.91%, respectively in Swiss albino mice, which has been intraperitoneally administrated with acetic acid [5]. On the basis of these results it can be suggested thatM. paniculata bark and leaves extracts might possess analgesic and antinociceptive activity. Besides, according to Sharker et al.,M. paniculata extract indicated cytotoxic effects and it has to be taken into consideration as well [1].

Anticancer Activities

The major compound found inM. paniculata oil, (E)-caryophyllene, was found to posses cytotoxic activity against MDA-MB-231 (IC50= 31.6 μg/mL) and Hs 578T (IC50= 78.3 g/mL) human tumor cells [62].

As there are few studies on anticancer properties of M. paniculata, having a comparison of its chemical constituents with other plant extracts that have shown significant cytotoxic effects, might be useful as a clue for further investigations. A plant extract that is comparable to M. paniculata, is Juniperus phoenicea leaves and berries extract which is rich in the same Monoterpene hydrocarbons (e.g. Sabinene) that are also found inM. paniculata [24]. J. phoenicea extract has indicated significant cytotoxic activity against U251, HeLa, H460, HepG2 and MCF-7 cell lines.

Valko et al. investigated cytotoxic effect of water extracts from leaves and branches of Philadelphus coronaries L. (Hydrangeaceae) against A431 (human skin carcinoma cell line) and MCF-7 (human breast adenocarcinoma cell line), where both extracts from the leaves and branches showed significant cytotoxic effects against the two cancer cell lines. The cytotoxicity of these extracts might be due to the presences of umbelliferone and scopolin, two coumarins that were also found inM. paniculata extract [21].

Conclusion

Different medicinal potentials ofM. paniculata in various diseases have been reported by many investigators. However, there is a definite requirement of more detailed studies on the mechanisms of these properties. The current state of research onM. paniculata implicates great potential of the isolated bioactive compounds in treating diseases. With the advancement in medicinal chemistry and bioinformatics, the ethnomedicinal usage ofM. paniculata can be scientifically explained and proved through in vitro or in vivo studies and may consequently be developed as potential plant-based drugs.

References

  1. Sharker SM, Shahid IJ, Hasanuzzaman M (2009) Antinociceptive and bioactivity of leaves of Murraya paniculata (L.) Jack, Rutaceae. Brazilian Journal of Pharmacognosy 19: 746-748.
  2. Mondal SK Ray B, Ghosal PK, Teleman A, Vuorinen T (2001) Structural features of a water soluble gum polysaccharide from Murraya paniculata fruits. Int J Biol Macromol 29: 169-174.
  3. Chatterjee A, Pakrashi SC (2001) Treatise of Indian Medicinal Plants. NISC Delhi 1: 14.
  4. Gautam MK, Gupta A, Vijay Kumar M, et al. (2012) Studies on the hypoglycemic effects of Murrayapaniculata Linn. Extracton alloxan-induced oxidative stress in diabetic and non-diabetic models. APJTM 2: 186-191.
  5. Narkhede MB, Aimire PV, Wagh AE (2012) Evaluation of antinociceptive and anti-inflammatory activities of ethanol extract of Murraya paniculata leaves in experimental rodents. IJPPS 4: 247-251.
  6. Rohman A, Sugeng R (2005) Antioxidant potency of ethanolic extract of Kemuning leaves (Murraya paniculata (L) Jack) in vitro. Majalah Farmasi Indonesia 16: 136-140.
  7. Zhang JY, Li N, Che YY, Zhang Y, Liang SX, et al. (2011) Characterization of seventy polymethoxylated flavonoids (PMFs) in the leaves of Murraya paniculata by on-line high-performance liquid chromatography coupled to photodiode array detection and electrospray tandem mass spectrometry. J Pharm Biomed Anal 56: 950-961.
  8. Podder MK, Das BN, Saha A, Ahmed M (2011) Analgesic activity of bark of Murraya paniculata. IJMMS 3: 105-108.
  9. Proenca Barros FA, Rodrigues-Filho E (2005) Four spiroquinazoline alkaloids from Eupenicillium sp. isolated as an endophytic fungus from leaves of Murraya paniculata (Rutaceae). Biochemical Systematics and Ecology 33: 257-268.
  10. Li Q, Zhu LF, But PPH, Kong YC, Chang HT, Waterman PG (1988) Monoterpene and sesquiterpene rich oils from the leaves of Murraya species: chemotaxonomic signi?cancer. Biochemical Systematics and Ecology 16: 491–494.
  11. Ito C, Furukawa H, Ishii H, Ishikawa T, Haginiwa J (1990) The chemical composition of Murraya paniculata. The structure of five new coumarins and one new alkaloid and the stereochemistry of murrangatin and related coumarins. J Chem Soc Perkin Trans 1: 2047-2055.
  12. Kinoshita T, Firman K (1997) Myricetin 5,7,3',4',5'-pentamethyl ether and other methylated flavonoids from Murraya paniculata. Phytochemistry 45: 179-181.
  13. Ferracin RJ, da Silva MGF, Fernandes JB, Vieira PC (1998) Flavonoids from the fruits of Murraya paniculata. Phyrochemistroy 47: 393-396.
  14. Kong YC, Ng KH, Wat CKH, Wong A, Saxena LF, et al. (1985) Yuehchukene - a novel anti-implantation indole alkaloid from Murraya paniculata. Planta Medica 49: 304-307.
  15. Wu TS, Liou MJ, Jong TT, Chen YJ, Lai JS (1989) Indole alkaloids and coumarins from the root bark of Murraya paniculata var. omphalocarpa. Phytochemistry 28: 2873-2874.
  16. Wu TS, Chan YY, Leu YL, Huang SC (1994) A flavonoid and indole alkaloid from flowers of Murraya paniculata. Phytochemistry 37: 287 -288.
  17. Silva LB, Silva ULL, Mahendran M, Jennings RC (1980) Flavonoids of Murraya paniculata (Linn.) Jack. Journal of the National Science Council of Sri Lanka 8: 123-125.
  18. Yang JS, Du MH (1984) Studies on the constituents of Murraya paniculata (L.) Jack grown in Yunnan. Acta Botanica Sinica 26: 184-188.
  19. Kinoshita T, Firman K (1996) Highly oxygenated flavonoids from Murraya paniculata. Phytochemistry 42: 1207-1210.
  20. Ferracin RJ, Silva MF, Fernandes JB, Vieira PC (1998) Flavonoids from the fruits of Murraya paniculata. Phytochemistry 47: 393-396.
  21. Lin JK, Wu TS (1994) Constituents of flowers of Murraya paniculata. J. Chinese Chem. Soc. 41: 213-216.
  22. Kinoshita T, Wu JB, Ho FC (1996) The isolation of a prenylcoumarin of chemotaxonomic significance from Murraya paniculata var. omphalocarpa. Phytochemistry 43: 125-128.
  23. Rahman AU, Sharbbir M, Sultan SSZ, Jabbar A, Choudhary MI, (1997) Cinnamate and coumarins from the leaves of Murraya paniculata. Phytochemistry 44: 683-685.
  24. Chowdhury JU, Bhuiyan NI, Yusof M (2008) Chemical composition of the leaf essential oils of Murraya koenigii (L.) Spreng and Murraya paniculata (L.) Jack. Bangladesh J Pharmacol 3: 59-63.
  25. Rout PK, Rao YR, Sree A, Naik SN (2007) Composition of essential oil, concrete, absolute, and wax and headspace volatiles of Murraya paniculata (Linn.) Jack flowers. FLAVOUR FRAG J 22: 352-357.
  26. Kishikawa N, Ohyama K, Yao J, Miyamoto A, Imazato T, et al. (2010) Automated analysis of the serum antioxidative activities against five different reactive oxygen species by sequential injection system with a chemiluminescence detector. Clin Chim Acta 411: 1111-1115.
  27. Rao PS, Kalva S, Yerramilli A, (2011) Free radicals and tissue damage: role of antioxidants. Free Rad Antiox 1: 2-7.
  28. May MJ, Madge LA (2007) Caspase inhibition sensitizes inhibitor of NF-kappaB kinase beta-deficient fibroblasts to caspase-independent cell death via the generation of reactive oxygen species. J Biol Chem 282: 16105-16116.
  29. Paydar M, Moharam BA, Wong YL, Looi CY, Wong WF, et al. (2013) Centratherum anthelminticum (L.) Kuntze a potential medicinal plant with pleiotropic pharmacological and biological activities. Int. J. Pharm. 9: 211-226.
  30. Braida I, Mattea M, Cardarelli D (2008) Extraction–adsorption–desorption process under supercritical conditionas a method to concentrate antioxidants from natural sources. J of Supercritical Fluids 45: 195–199.
  31. Paydar M, Wong YL, Moharam BA, Wong WF, Looi CY (2013) In vitro anti-oxidant and anti-cancer activity of methanolic extract from Sanchezia speciosa leaves. Pak J Biol Sci 16: 1212-1215.
  32. Moghadamtousi SZ, Kadir HA, Paydar M, Rouhollahi E, Karimian H (2014) Annona muricata leaves induced apoptosis in A549 cells through mitochondrial-mediated pathway and involvement of NF-κB. BMC Complement Altern Med 14: 299.
  33. Upadhyay G, Gupta SP, Prakash O, Singh MP (2010) Pyrogallol-mediated toxicity and natural antioxidants: triumphs and pitfalls of preclinical findings and their translational limitations. Chem Biol Interact 183: 333-340.
  34. Paydar M, Kamalidehghan B, Wong YL, Wong WF, Looi CY, et al. (2014) Evaluation of cytotoxic and chemotherapeutic properties of boldine in breast cancer using in vitro and in vivo models. Drug Des Devel Ther 8: 719-733.
  35. Zhang JY, Lu JQ, Gao XY (2013) Characterization of thirty-nine polymethoxylated flavonoids (PMFs) in the branches of Murraya paniculata by HPLC-DAD-ESI-MS/MS. Chinese Journal of Natural Medicines 11: 63-70.
  36. Anagnostopoulou MA, Kefalas P, Kokkalou E, Assimopoulou AN, Papageorgiou VP (2005) Analysis of antioxidant compounds in sweet orange peel by HPLC-diode array detection-electrospray ionization mass spectrometry. Biomed Chromatogr 19: 138-148.
  37. Chen CH, Chan HC, Chu YT, Ho HY, Chen PY, et al. (2009) Antioxidant activity of some plant extracts towards xanthine oxidase, lipoxygenase and tyrosinase. Molecules 14: 2947-2958.
  38. Shaikh A, Choudhary MI (2011) Bioassay studies of 2'-O-ethylmurrangatin isolated from a medicinal plant, Murrayapaniculata. Turk J Biol 35: 751-755.
  39. Gual P, Le Marchand-Brustel Y, Tanti J (2003) Positive and negative regulation of glucose uptake by hyperosmotic stress. Diabetes Metab 29: 566-575.
  40. Tiong SH, Looi CY, Hazni H, Arya A, Paydar M, et al. (2013) Antidiabetic and antioxidant properties of alkaloids from Catharanthus roseus (L.) G. Don. Molecules 18: 9770-9784.
  41. Cazarolli LH, Pereira DF, Kappel VD, Folador P, Figueiredo Mdos S, et al. (2013) Insulin signaling: a potential signaling pathway for the stimulatory effect of kaempferitrin on glucose uptake in skeletal muscle. Eur J Pharmacol 712: 1-7.
  42. Yamamoto T, Tanaka S, Suga S, Watanabe S, Nagatomo K, et al. (2011) Syntheses of 2-NBDG analogues for monitoring stereoselective uptake of D-glucose. Bioorg Med Chem Lett 21: 4088-4096.
  43. Taha H, Arya A, Paydar M, Looi CY, Wong WF, et al. (2014) Upregulation of insulin secretion and downregulation of pro-inflammatory cytokines, oxidative stress and hyperglycemia in STZ-nicotinamide-induced type 2 diabetic rats by Pseuduvaria monticola bark extract. Food Chem Toxicol 66: 295-306.
  44. Veluthakal R, Suresh MV, Kowluru A (2009) Down-regulation of expression and function of nucleoside diphosphate kinase in insulin-secreting beta-cells under in vitro conditions of glucolipotoxicity. Mol Cell Biochem 329: 121-129.
  45. Henquin JC (2011) The dual control of insulin secretion by glucose involves triggering and amplifying pathways in ß-cells. Diabetes Res Clin Pract 93 Suppl 1: S27-31.
  46. Kalsekar ID, Madhavan SS, Amonkar MM, Douglas SM, Makela E, et al. (2006) Impact of depression on utilization patterns of oral hypoglycemic agents in patients newly diagnosed with type 2 diabetes mellitus: a retrospective cohort analysis. Clin Ther 28: 306-318.
  47. Mukherjee PK, Maiti K, Mukherjee K, Houghton PJ (2006) Leads from Indian medicinal plants with hypoglycemic potentials. J Ethnopharmacol 106: 1-28.
  48. Aragão DM, Guarize L, Lanini J, da Costa JC, Garcia RM, et al. (2010) Hypoglycemic effects of Cecropia pachystachya in normal and alloxan-induced diabetic rats. J Ethnopharmacol 128: 629-633.
  49. Yongri J, Dayuan S, Xuwen L (2008) Applications of murraya jasmine or age leaf total flavones in preparation of medicament for curing diabetes. Changchun Ruide Pharmaceutical Technology.
  50. Paydar M, Teh CSJ, Thong KL (2013) Prevalence and characterization of potentially virulent Vibrio parahaemolyticus in seafoods in Malaysia using conventional methods, PCR and REP-PCR. Food Conrol 32: 13-18.
  51. Shawish HB, Paydar M, Looi CY, Wong YL, Movahed E, et al. (2013) Nickel(II) complexes of polyhydroxybenzaldehyde N4-thiosemicarbazones: synthesis, structural characterization and antimicrobial activities. Transition Metal Chemistry.
  52. Sakunpak A, Panichayupakaranant P (2012) Antibacterial activity of Thai edible plants against gastrointestinal pathogenic bacteria and isolation of a new broad spectrum antibacterial polyisoprenylated benzophenone, chamuangone. Food Chem 130: 826-831.
  53. da Rocha AB, Lopes RM, Schwartsmann G (2001) Natural products in anticancer therapy. Curr Opin Pharmacol 1: 364-369.
  54. Azizi SSSA, Sukari MA, Rahmani M, Kitajima M, Ahpandi NJ (2010) Coumarins from Murraya paniculata (Rutaceae). The Malaysian Journal of Analytical Sciences 14: 1-5.
  55. Salawu SO, Ogundare AO, Ola-Salawu BB, Akindahunsi AA (2011) Antimicrobial activities of phenolic containing extracts of some tropical vegetables. Afric J Pharm Pharmacol 5: 486-492.
  56. Hendra R, Ahmad S, Sukari A, Shukor MY, Oskoueian E (2011) Flavonoid analyses and antimicrobial activity of various parts of Phaleria macrocarpa (Scheff.) Boerl fruit. Int J Mol Sci 12: 3422-3431.
  57. Gupta M, Mazumder UK, Gomathi P, Selvan VT (2006) Antiinflammatory evaluation of leaves of Plumeria acuminata. BMC Complement Altern Med 6: 36.
  58. Ghani A (1998) Medicinal Plants of Bangladesh. The Asiatic Society of Bangladesh, Dhaka, Bangladesh.
  59. Whittle BA (1964) The Use of Changes in Capillary Permeability in Mice to Distinguish between Narcotic and Nonnarcotic Alalgesics. Br J Pharmacol Chemother 22: 246-253.
  60. Chevallier A (1996) The Encyclopedia of medicinal plants. (1stedn) DK publishing Inc. New York.
  61. El-Sawi SA, Motawae HM, Ali AM (2007) Chemical Composition, Cytotoxic Activity and Antimicrobial Activity of Essential Oils of Leaves and Berries of Juniperus phoeniceal Grown in Egypt. Afr J Tradit Complement Altern Med. 4: 417-426.
  62. Valko V, Fickova M, Pravdova E, Nagy M, Grancai D, et al. (2006) Cytotoxicity of Water Extracts from Leaves and Branches of Philadelphus coronarius L. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 150: 71-73.
Citation: Sayar K, Paydar M, Pingguan-Murphy B (2014) Pharmacological Properties and Chemical Constituents of Murraya paniculata (L.) Jack. Med Aromat Plants 3:173.

Copyright: © 2014 Sayar, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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