Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biol
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Sperm Cryopreservation: The Complete Guide to Preservation and Fertility Options

Haibo Tang^{* *}Correspondence: Haibo Tang, Department of Andrology, Tohoku University, Sendai, Japan, Email:

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Description

Sperm cryopreservation, or sperm freezing, is a medical technique that allows sperm to be preserved at very low temperatures for future use. This process is an essential component of Assisted Reproductive Technologies (ART) such as In Vitro Fertilization (IVF), Intrauterine Insemination (IUI), and sperm donation. It also provides a valuable option for men who may face fertility challenges due to medical treatments, aging, or personal circumstances. This article analyze the process of sperm cryopreservation, its uses, benefits, and considerations.

What is sperm cryopreservation?

Sperm cryopreservation is the process of freezing sperm cells to preserve them for later use. By cooling sperm to extremely low temperatures, typically -196°C (-320.8°F), the cells remain viable for long periods, even for many years. When the sperm is needed, it can be thawed and used in fertility treatments, such as IVF or IUI, or for sperm donation. The freezing process involves isolating sperm from the semen, diluting the sperm with a special freezing medium to protect it from damage during freezing, and then storing it in liquid nitrogen tanks. Sperm cryopreservation is a safe and widely used method in reproductive medicine and has helped countless individuals and couples conceive when faced with fertility challenges [1].

Why is sperm cryopreservation used?

Sperm cryopreservation is used in a variety of situations, ranging from medical treatments to personal decisions about family planning. The most common reasons for sperm freezing include:

Fertility preservation before medical treatments: Certain medical treatments, such as chemotherapy or radiation therapy, can negatively impact male fertility by damaging sperm production. For men diagnosed with cancer or other conditions that require aggressive treatments, sperm cryopreservation offers a way to preserve fertility before undergoing these procedures [2].

Vasectomy or fertility preservation before surgery: Men who undergo a vasectomy, a surgical procedure to prevent pregnancy by cutting the vas deferens (the tubes that carry sperm), may choose to freeze sperm before the procedure in case they wish to have children later [3]. Sperm cryopreservation ensures that they have sperm available for fertility treatments even if they later experience difficulty with natural conception.

Sexual dysfunction or injury: Men who experience sexual dysfunction, spinal cord injuries, or other conditions that impact the ability to ejaculate may use sperm cryopreservation as a means of preserving their fertility [4]. In such cases, sperm can be collected through techniques like Testicular Sperm Extraction (TESE) or electro ejaculation and then frozen for future use.

The sperm cryopreservation process

The process of sperm cryopreservation involves several steps to ensure that sperm cells remain viable after being frozen. Here is an overview of the procedure:

Sperm collection: The first step in sperm cryopreservation is sperm collection. A sample of semen is typically obtained through masturbation into a sterile container in a clinic or sperm bank [5]. For men who cannot ejaculate normally, sperm can also be collected through medical procedures like electro ejaculation or TESE (Testicular Sperm Extraction).

Sperm analysis: Once the sample is collected, a laboratory technician analyzes the sperm to evaluate the quantity, motility (movement), morphology (shape), and overall quality [6]. This information helps to determine whether sperm freezing is a good option and if any further steps, such as sperm washing or concentration, are needed.

Freezing process (cryopreservation): After analysis, the sperm is diluted with a cryoprotectant solution, which protects the sperm from ice crystal formation that can occur during freezing [7]. This cryoprotectant helps prevent damage to the sperm during the freezing and thawing process. The sperm is then frozen in liquid nitrogen at a temperature of -196°C (-320.8°F), ensuring that the sperm remains viable for long-term storage.

Risks and considerations

While sperm cryopreservation has a high success rate, it is not without risks and considerations:

Sperm viability after thawing: Not all sperm may survive the freezing and thawing process. In some cases, sperm may lose motility or fail to fertilize an egg [8].

Cost: The process of sperm cryopreservation, including storage and maintenance fees, can be expensive. However, for men undergoing medical treatments or facing infertility, the cost may be a necessary investment [9].

Limited success rates: Although sperm freezing can help preserve fertility, it is important to understand that it does not guarantee successful conception [10]. Other factors, such as the quality of the eggs and the woman’s reproductive health, also play a significant role in fertility outcomes.

Conclusion

Sperm cryopreservation is a valuable tool for men seeking to preserve their fertility for future use. Whether for medical reasons, advanced age, or personal choices, the ability to freeze sperm offers flexibility and options in family planning. While there are costs and risks associated with the process, it provides a viable solution for men experiencing fertility challenges, and it has played an important role in helping many individuals and couples achieve their reproductive goals. If you're considering sperm cryopreservation, consulting with a fertility specialist can provide personalized advice and help you understand your options.

References

1. Ollero M, Perez-Pe R, Muino-Blanco T, Cebrian-Perez JA. Improvement of ram sperm cryopreservation protocols assessed by sperm quality parameters and heterogeneity analysis. Cryobiology. 1998 1;37(1):1-2.

   [Crossref] [Google Scholar] [PubMed]

2. Nijs M, Ombelet W. Cryopreservation of human sperm. Hum Fertil (Camb). 2001 1;4(3):158-163.

   [Crossref] [Google Scholar] [PubMed]

3. Yang H, Carmichael C, Varga ZM, Tiersch TR. Development of a simplified and standardized protocol with potential for high-throughput for sperm cryopreservation in zebrafish Danio rerio. Theriogenology. 2007 15;68(2):128-136.

   [Crossref] [Google Scholar] [PubMed]

4. Baert Y, Van Saen D, Haentjens P, In't Veld P, Tournaye H, Goossens E. What is the best cryopreservation protocol for human testicular tissue banking?. Hum Reprod. 2013 1;28(7):1816-1826.

   [Crossref] [Google Scholar] [PubMed]

5. Li YX, Zhou L, Lv MQ, Ge P, Liu YC, Zhou DX. Vitrification and conventional freezing methods in sperm cryopreservation: A systematic review and meta-analysis. Eur J Obstet Gynecol Reprod Biol. 2019 1;233:84-92.

   [Crossref] [Google Scholar] [PubMed]

6. Salazar Jr JL, Teague SR, Love CC, Brinsko SP, Blanchard TL, Varner DD. Effect of cryopreservation protocol on postthaw characteristics of stallion sperm. Theriogenology. 2011 1;76(3):409-418.

   [Crossref] [Google Scholar] [PubMed]

7. BELL M, Wang RU, HELLSTROM WJ, SIKKA SC. Effect of cryoprotective additives and cryopreservation protocol on sperm membrane lipid peroxidation and recovery of motile human sperm. J Androl. 1993 12;14(6):472-488.

   [Crossref] [Google Scholar] [PubMed]

8. Cross NL, Hanks SE. Effects of cryopreservation on human sperm acrosomes. Hum Reprod. 1991 1;6(9):1279-1283.

   [Crossref] [Google Scholar] [PubMed]

9. Forero-Gonzalez RA, Celeghini EC, Raphael CF, Andrade AF, Bressan FF, Arruda RP. Effects of bovine sperm cryopreservation using different freezing techniques and cryoprotective agents on plasma, acrosomal and mitochondrial membranes. Andrologia. 2012; 44:154-169.

   [Crossref] [Google Scholar] [PubMed]

10. Liu T, Gao J, Zhou N, Mo M, Wang X, Zhang X,et al. The effect of two cryopreservation methods on human sperm DNA damage. Cryobiology. 2016 1;72(3):210-225.

    [Crossref] [Google Scholar] [PubMed].