Applied Microbiology: Open Access
Open Access

The Insights and Implications of Microbial Physiology in Human Health

Akihiko Morotoka^{* *}Correspondence: Akihiko Morotoka, Department of Medicine, Kumamoto University, Kumamoto, Japan, Email:

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Description

Microbial physiology, the study of how microorganisms function and adapt to their environments, lies at the intersection of microbiology, biochemistry, and ecology. While often overshadowed by the allure of molecular genetics and biotechnology, understanding microbial physiology is indispensable for diverse fields, including medicine, agriculture, and biotechnology [1].

Metabolic versatility

Microorganisms exhibit unparalleled metabolic diversity, enabling them to thrive in a wide range of habitats, from extreme environments like deep-sea hydrothermal vents to the human gut. Central to microbial physiology is the concept of catabolism, the breakdown of complex molecules into simpler ones to generate energy and building blocks for cellular processes [2]. Microbes employ diverse metabolic pathways, from glycolysis and oxidative phosphorylation to anaerobic fermentation and chemolithotrophy, tailored to their ecological niches. Recent advances in omics technologies have unveiled novel metabolic pathways and regulatory mechanisms, shedding light on the metabolic plasticity of microorganisms and their role in biogeochemical cycles [3].

Environmental adaptations

Microbial physiology is intricately linked to environmental conditions, driving adaptive responses to fluctuations in temperature, pH, nutrient availability, and osmolarity. psychrophilic thrive in cold environments, with enzymes adapted to low temperatures, while thermophiles flourish in hot springs, boasting heat-stable proteins. Halophiles thrive in saline habitats, employing compatible solutes to maintain osmotic balance, while acidophiles and alkaliphiles thrive in acidic and alkaline environments, respectively, by maintaining intracellular pH homeostasis. Understanding these adaptive mechanisms not only elucidates fundamental aspects of microbial physiology but also informs biotechnological applications, such as industrial biocatalysis and bioremediation [4].

Host-microbe interactions

Microbial physiology plays a pivotal role in host-microbe interactions, shaping the outcome of symbiotic, commensal, and pathogenic relationships [5]. In the human microbiome, microbial communities influence host physiology through metabolic interactions, modulating immune responses, nutrient metabolism, and even behavior. Pathogenic microorganisms exploit host resources and evade immune surveillance by modulating virulence factors and metabolic pathways, leading to infectious diseases. Conversely, probiotic microorganisms confer health benefits by producing antimicrobial compounds, competing with pathogens for resources, and modulating host immune function. Deciphering the intricate exchange between microbial physiology and host physiology holds promise for developing novel therapeutics and interventions to treat infectious and inflammatory diseases [6].

Biofilm formation and persistence

Biofilms, multicellular communities of microorganisms encased in a self-produced matrix, represent a quintessential example of microbial physiology in action. Biofilm formation is a dynamic process orchestrated by microbial communication, metabolic cooperation, and extracellular matrix production [7]. Within biofilms, microorganisms exhibit distinct physiological states, with cells in the outer layers metabolizing nutrients and interacting with the external environment, while cells in the inner layers experience nutrient limitation and metabolic quiescence. This spatial organization confers resilience to environmental stresses, including antibiotics and host immune defenses, contributing to chronic infections and device-associated infections. Understanding the physiological heterogeneity within biofilms is crucial for developing strategies to disrupt biofilm formation and enhance antimicrobial efficacy [8].

Biotechnological applications

Microbial physiology promotes numerous biotechnological applications, from industrial fermentation and bioremediation to the production of biofuels and pharmaceuticals. By harnessing the metabolic capabilities of microorganisms, biotechnologists can engineer microbial strains for enhanced productivity, substrate utilization, and product specificity [9]. Metabolic engineering techniques enable the manipulation of metabolic pathways to optimize desired outputs, such as the production of bio-based chemicals, enzymes, and biopolymers [10]. Moreover, synthetic biology approaches empower researchers to design novel metabolic pathways and cellular functions, paving the way for biobased solutions to global challenges, including sustainable agriculture, renewable energy, and environmental conservation [11].

Microbial physiology encompasses the diverse mechanisms by which microorganisms function and adapt to their environments, driving ecological processes, host-microbe interactions, and biotechnological innovations. Recent advancements in omics technologies, imaging techniques, and computational modeling have revolutionized our understanding of microbial physiology, unveiling new metabolic pathways, regulatory networks, and ecological interactions [12].

References

1. Takahashi M, Tanzawa K, Takahashi S. Adenophostins, newly discovered metabolites of Penicillium brevicompactum, act as potent agonists of the inositol 1, 4, 5-trisphosphate receptor. J Biol Chem. 1994;269(1):369-372.

   [Crossref] [Google Scholar] [PubMed]

2. Gordon JI, Klaenhammer TR. A rendezvous with our microbes. Proc Natl Acad Sci U S A. 2011;108(1):4513-4515.

   [Crossref] [Google Scholar] [PubMed]

3. Ozer T, Henry CS. based analytical devices for virus detection: Recent strategies for current and future pandemics. Trends Analyt Chem. 2021;144:116424.

   [Crossref] [Google Scholar] [PubMed]

4. Krysinski EP, Heimsch RC. Use of enzyme-labeled antibodies to detect Salmonella in foods. Appl Environ Microbiol. 1977;33(4):947-954.

   [Crossref] [Google Scholar] [PubMed]

5. Mak WC, Beni V, Turner AP. Lateral-flow technology: From visual to instrumental. Trends Analyt Chem. 2016;79:297-305.

   [Crossref] [Google Scholar]

6. Schröder U. Discover the possibilities: microbial bioelectrochemical systems and the revival of a 100-year–old discovery. J Solid State Electrochem. 2011;15:1481-1486.

   [Crossref] [Google Scholar]

7. Hongo M, Iwahara M. Application of electro-energizing method to L-glutamic acid fermentation. Agric Biol Chem. 1979;43(10):2075-2081.

   [Crossref] [Google Scholar]

8. García-Jiménez B, Torres-Bacete J, Nogales J. Metabolic modelling approaches for describing and engineering microbial communities. Comput Struct Biotechnol J. 2021;19:226-246.

   [Crossref] [Google Scholar] [PubMed]

9. Zaramela LS, Moyne O, Kumar M, Zuniga C, Tibocha-Bonilla JD, Zengler K. The sum is greater than the parts: exploiting microbial communities to achieve complex functions. Curr Opin Biotechnol. 2021; 67:149-157.

   [Crossref] [Google Scholar] [PubMed]

10. Wu S, Qiao J, Yang A, Liu C. Potential of orthogonal and cross-talk quorum sensing for dynamic regulation in cocultivation. Chem Eng J. 2022;445:136720.

    [Crossref] [Google Scholar]

11. Flemming HC, Wingender J, Szewzyk U, Steinberg P, Rice SA, Kjelleberg S. Biofilms: an emergent form of bacterial life. Nat Rev Microbiol. 2016;14(9):563-575.

    [Crossref] [Google Scholar] [PubMed]

12. Vrancken G, Gregory AC, Huys GR, Faust K, Raes J. Synthetic ecology of the human gut microbiota. Nat Rev Microbiol. 2019;17(12):754-763.

    [Crossref] [Google Scholar] [PubMed]