The pharmaceutical investigation is a part of down to earth science that includes a progression of procedure for recognizable proof, assurance, measurement and cleaning of a substance, the partition of the segments of an answer or blend, or assurance of structure of synthetic compounds. The substance might be a solitary compound or a blend of mixes and it might be in any of the dose structures. The substance utilized as pharmaceuticals are creatures, plants, miniaturized scale living beings, minerals, and different engineered items. The example to be examined is called as investigating and based on the size of the test, they can be delegated macro(0.1 g or more), semi miniaturized scale (0.01 g to 0.1 g), micro(0.001 g to 0.01 g), sub small scale (0.0001 g to 0.001 g), ultra-micro (underneath 10-4 g), follow analysis(100 to 10000 ppm). Among all, the semi miniaturized scale investigation is generally utilized. The subjective investigation is performed to build up the structure of regular/engineered substances. These tests are performed to demonstrate whether the substance or compound is available in the example or not. Different subjective tests are the discovery of developed gas, arrangement of hastens, limit tests, shading change responses, dissolving point, and breaking point test, etc. Quantitative investigative procedures are for the most part used to measure any compound or substance in the example. These strategies are situated in (a) the quantitative presentation of appropriate synthetic response and either estimating the measure of reagent added to finish the response or estimating the measure of response item acquired, (b) the characteristic development of a substance through a characterized medium under controlled conditions, (c) electrical estimation, (d) estimation of some spectroscopic properties of the compound.