Journal of Clinical Toxicology
Open Access
ISSN: 2161-0495
+44 1478 350008
ISSN: 2161-0495
+44 1478 350008
Zuyue Sun,Xiang Meng, Juan Jiang, Liming Chong, Yongwei Luo, Ting Zhang, Ying Li, Qi Pan, Yang Yang, Sichong Xu and Li Zhou
Shanghai Institute of Planned
Parenthood Research, China
Posters & Accepted Abstracts: J Clinic Toxicol
Objective: The objective of this study was to determine the toxicokinetic profile of
curdione in pregnant SD rats as well as the transference of curdione into the fetus through
the placental barrier system using LC-MS/MS.
Methods: A sensitive analytical method for determining the plasma concentration of
curdione was developed and applied in the determination of curdione in pregnant SD rats
as a simulated model. Thirteen pregnant SD rats were treated with 7, 21 and 63 mg/kg
curdione once daily from GD6 to GD15. Blood samples were collected at different time
points on GD6 and GD15. Maternal plasma, placental plasma, placenta tissue, amniotic
fluid and fetal tissue were collected for concentration analysis after all the animals were
sacrificed following one repeated dose.
Results: The results indicated that Cmax, AUC (0-t) and AUC (0-�??) increased in a dosedependent
manner both on GD6 and GD15. At 7 mg/kg group, the total serum clearance
value on GD15 was reduced to approximately 16.4% of that on GD6, and the volume of
distribution was also significantly decreased (p<0.05). Curdione could be detected in the
maternal plasma, placental plasma, placenta tissue, amniotic fluid and fetal tissue, and its
concentration in the fetal tissue reached saturation at 21 mg/kg.
Conclusion: It presents with the risk of toxic accumulation in the concomitant toxicokinetics
of reproductive toxicity studies in SD rats and it may affect the fetus via transference
through the placental barrier system.
Zuyue Sun is the Research Director of National Evaluation Centre for the Toxicology of Fertility Regulating Drug; a laboratory has acquired the GLP certificate of CFDA (China Food and Drug Administration). He has published more than 312 theses in China and abroad reputed journals, and 258 study projects being carried out in his laboratory.
E-mail: Sunzy64@163.com