Orthopedic & Muscular System: Current Research
Open Access

Aacacetin inhibits expression of matrix metalloproteinases in interleukin 1β-stimulated fibroblastlike synoviocytes

3^rd International Conference and Exhibition on Orthopedics & Rheumatology

July 28-30, 2014 DoubleTree by Hilton Hotel San Francisco Airport, USA

Li-Dong Wu, Wei-Ping Chen, Zhi-Gao Yang, Peng-Fei Hu and Jia-Peng Bao

Accepted Abstracts: Orthop Muscul Syst

Abstract :

I t is well known that Rheumatoid arthritis (RA) is an autoimmune joint disease in which fibroblast-like synoviocytes (FLSs) play a pivotal role. In the present study, it was investigated the anti-arthritic properties of acacetin in FLSs. The expression of matrix metalloproteinase (MMP)-1, MMP-3, and MMP-13 were investigated by quantitative real-time polymerase chain reaction (qRT-PCR) and western-blot at gene and protein levels. At the same time, the phosphorylation of mitogen-activated protein kinases (MAPK) was investigated. The DNA binding activity of NF-κB was investigated by electrophoretic mobility shift assay. For the in vivo study, mouse received intragastric administration of acacetin, the symptoms of arthritis in vivo and the histopathological assessment of joints were performed. It was found that acacetin exerted anti-arthritic properties in an adjuvant-induced arthritis mouse model. Obtained data suggest that acacetin possess potential value in the treatment of RA.