Translational Medicine
Open Access
ISSN: 2161-1025
+44 1223 790975
ISSN: 2161-1025
+44 1223 790975
Bing Zhu
The first Affiliated Hospital of Bengbu Medical College, China
Posters & Accepted Abstracts: Transl Med
Background: Chemokines have been recognized as important modulators of angiogenesis and they play critical roles in the development and metastasis of hepatocellular carcinoma (HCC), although their origins and latent molecular mechanisms remain elusive. Aim: The aim of this study was to investigate how activated hepatic stellate cells (a-HSCs) promote angiogenesis in HCC. Methods: A total of 22 HCC patients were enrolled randomly. We used immunohistochemistry; western blotting and enzyme-linked immunosorbent assay (ELISA) to analyze the production of interleukin-8 (IL-8) in a-HSCs derived from HCC tissues. The angiogenic effects of IL-8 in vitro and in vivo were assessed by ELISA, real-time quantitative polymerase chain reaction, capillary tube formation assay and chick embryo chorioallantoic membrane assay. Results: The present study showed that IL-8 was enriched predominantly in the tumor stroma of HCC tissues and was mainly derived from a-HSCs, rather than from hepatoma cells, in vivo and in vitro. Angiogenesis was most active at the invading edge, which was close to the a-HSCs. The angiogenic effect was dramatically attenuated by an IL-8 neutralizing antibody both in vitro and in vivo. Moreover, the IL-8 neutralizing antibody down-regulated Ser727-phosphorylated STAT3 levels in hepatoma cells treated with a-HSCs conditioned medium. Conclusions: These findings reveal that a-HSCs within the stroma of HCC contribute to tumor angiogenesis via IL-8.
Bing Zhu is a Doctor and has more than 10 years of experience in General Surgery department. He has completed his PhD from Zhongshan University's third affiliated hospital. Currently, his major directions of research are Tumor Angiogenesis.
Email: bbmczhubing@163.com