Journal of Chromatography & Separation Techniques
Open Access

Adsorptive removal of Triton X-100 from human plasma and its derivatives

4^th World Congress on Chromatography

August 07-09, 2017 | Rome, Italy

Gargi Redkar and Sandeep Kale

DBT-ICT-Centre for Energy Biosciences, India
Institute of Chemical Technology, Maharashtra, India

Posters & Accepted Abstracts: J Chromatogr Sep Tech

Abstract :

Viral transmission during the use of human plasma and its derivatives to treat various medical conditions can be fatal. Solvent/ detergent treatment using non-ionic detergents like Triton X-100 inactivate the lipid enveloped viruses. However, the detergent interferes with downstream processing and analysis. Also, WHO permits a residual level of <25 ppm of Triton X-100 and thus it needs to be removed from post viral inactivation. Removal of Triton X-100 poses a challenge due to its low CMC and non-ionic character. Selective removal of Triton X-100 was studied using various hydrophobic resins screened on the basis of adsorption capacity, uptake kinetics and effect of plasma proteins on these parameters. Resins showing higher adsorption capacity and uptake rate with lower protein binding were selected for column studies. Breakthrough capacity of the shortlisted resins was determined at different flow rates and concentrations along with the effect of proteins. A simple and sensitive HPLC method was developed to detect Triton X-100 in the treated samples at ppm level. This research work asserted the impact of various resin characteristics and plasma proteins on selective detergent removal and thus the mechanism of adsorption of Triton X-100 onto these resins.

Biography :

Email: sb.kale@ictmumbai.edu.in