Journal of Glycobiology

Journal of Glycobiology
Open Access

ISSN: 2168-958X

+44 1478 350008

Alzheimer’s disease rats, inhibit β-amyloid fibril formation


Glycobiology World Congress

August 10-12, 2015 Philadelphia, USA

Maria O Longas

Scientific Tracks Abstracts: J Glycobiol

Abstract :

Alzheimer’s disease rats were developed by Taconic Biosciences, MPA. Development took 5 weeks. After this time, each
of the 6 Alzheimer’s disease rats was injected with NaH (3 mg/3 mL sterilized phosphate buffer saline, SPBS); 3 grams
of NaH/gram of Rat body mass were used for injection. The controls were normal Rats, 6 of which were injected with 3 mL
of SPBS per gram of Rat body mass. After 5 weeks, all rats were guillotined; the heads were removed and stored at -35o C
for tissue culture. Cells were cultured following conditions described for human Alzheimer’s disease cells and carried out in
6-well-tissue-culture-dishes each containing 4 mL of the following media: Astrocyte Basal Medium from Lonza adjusted to
15% (w/w) fetal bovine serum and fortified with L-Glutamine (5 mL), Ascorbic Acid (0.5 mL), Epidermal Growth Factors
(1.25 mL), Insulin (0.5 mL) and Gentamicin Sulfate Amphotencin-β (5 mL). Cells grew in these media at 37o C under 5% CO2
for 7 days. They were then stained with Congo Red. Figure IIIR displays Alzheimer’s disease hippocampus cells with plenty of
β-Amyloid tangles while Figure IVR has cells from the normal hippocampus cells with no tangle formation. NaH is therefore
effective to inhibit β-Amyloid tangle formation in Alzheimer’s disease.

Biography :

Maria Longas has completed her PhD at New York University and done her postdoctoral training at Columbia University School of Medicine with Dr. Karl Meyer. She is a full Professor
of Chemistry at Purdue University Calumet in Hammond, IN. She has more than 24 papers published in reputable journals, and has served as a reviewer for several journals.

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