Translational Medicine
Open Access

An update on methods for cryopreservation and thawing of hemopoietic stem cells

14^th Annual Conference on Translational Medicine and Oncologists Meet

November 28-30, 2016 San Francisco, USA

Lucilla Lecchi

Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Italy

Posters & Accepted Abstracts: Transl Med

Abstract :

This presentation will focus on a number of variables related to stem cells cryopreservation procedures of minimally manipulated products containing allogeneic or autologous hemopoietic progenitor cells (HPC) used for transplantation. The issues includes: regulations and standards, processing, process validation and qualification, volume reduction, cell concentration, volume, freezing, storage, cooling rate, warming events, quarantine, cross contamination during storage and thawing. New approaches of processing were developed such as automatic devices for volume reduction and high cell concentration in the frozen product. DMSO at 10% final concentration is still the most used cryo-protectant for HPC cryopreservation. Although controlled rate freezing is the recommended method for HPC cryopreservation, alternative methods may be used. Last generation vapor storage vessels ensure temperature stability better than older tanks and may reduce risks of cross-contamination. Finally, advantages and disadvantages of thawing procedures carried out at patient�s bedside or in the laboratory will be discussed.

Biography :

Email: llecchi@policlinico.mi.it