Journal of Chromatography & Separation Techniques
Open Access

Analysis of topiramate in human plasma using liquid chromatography - tandem mass spectrometry and its application to pharmacokinetic study

4^th World Congress on MASS SPECTROMETRY

June 19-21, 2017 London, UK

Kashyap N Thummar

Saurashtra University, India

Posters & Accepted Abstracts: J Chromatogr Sep Tech

Abstract :

A simple, sensitive and reliable liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of topiramate (TOP) in human plasma by using the liquid-liquid extraction method. The method was developed and validated over the linearity range 15-3000 ng/mL with 0.2 mL of human plasma using niclosamide (IS) as an internal standard. The MS-MS ion transitions were monitored at m/z 338.20-78.20 and m/z 325.20-171.20 for TOP and IS in negative mode respectively. Chromatographic separation was achieved on Gemini C18 (150 mmx4.6 mm, 5 �¼m) column with an isocratic mobile phase composed of 2 mM ammonium acetate and acetonitrile in the ratio of 15:85 (v/v), at a flow-rate of 0.5 mL/min. This method demonstrated intraand inter-day precision within 3.61-9.35% and 4.85-9.61% and accuracy within 93.09-105.24% and 92.46-104.40%. The extraction recoveries of TOP were over 89%. TOP was found to be stable throughout three freezeâ��thaw cycles, bench top and postoperative stability studies. The method is proved to be accurate and specific, and was successfully applied to the pharmacokinetic study in epileptic patients.

Biography :

Email: kasu_patel5@yahoo.co.in