Journal of Developing Drugs
Open Access

Antiangiogenic and AMP-activated protein kinase activities of Gracilaria coronopifolia J.G. Agardh extracts

2^nd International Conference and Exhibition on Pharmaceutical Development and Technology

May 11-12, 2018 Osaka, Japan

Katrin Mae M Ortega

University of Santo Tomas Graduate School, Philippines

Scientific Tracks Abstracts: J Dev Drugs

Abstract :

AMP-activated protein kinase (AMPK) is an intracellular energy sensor which is important in metabolic regulation, cell growth, and survival. Recently, dysfunction in AMPK is implicated to numerous angiogenesis-related diseases however, the mechanism remains elusive. In this study, the antiangiogenic activity of the red alga, Gracilaria coronopifolia, was determined through cellular signaling pathway of AMPK. Chorioallantoic membrane (CAM) assay showed that all extracts of G.coronopifolia inhibited angiogenesis in a dose-dependent manner. Among them, dichloromethane extract exhibited the most potent antiangiogenic activity (IC50=1.21 �¼g/mL, p=0.215) followed by hexane extract (IC50=3.08 �¼g/mL, p=0.479) and methanol extract (IC50=8.93 �¼g/mL, p=0.042). Antiangiogenic activity was correlated to a low concentration of Fe, Zn and Cu of duck CAM determined using flame atomic absorption spectrometer (fAAS) and colorimetric assay. Likewise, in vitro AMPK signaling assay showed that all extracts activated AMPK, with dichloromethane extract having the lowest EC50 of 70.2 �¼g/mL. Thin layer chromatography (TLC) and gas chromatography-mass spectrometry (GC-MS) revealed the active component of each extracts. Notably, this is the first report on the AMPK activity of G.coronopifolia related to new blood vessel formation and a colorimetric-based correlation of angiogenesis based on Fe, Zn and Cu concentration in the duck chorioallantoic membrane.

Biography :

Katrin Mae M Ortega has completed her Master of Science in Pharmacy from of University of Santo Tomas, Philippines.
Email:katrin.ortega24@gmail.com