Journal of Proteomics & Bioinformatics
Open Access

Antibodies are not required to a protective immune response against dengue virus elicited in a mouse encephalitis model

3^rd International Conference on Antibodies, Bio Therapeutics & B2B & Genetic and Protein Engineering

November 08-09, 2017 | Las Vegas, USA

Jaime Henrique Amorim

Universidade Federal do Oeste da Bahia, Brazil

Posters & Accepted Abstracts: J Proteomics Bioinform

Abstract :

Generating neutralizing antibodies have been considered a prerequisite to control dengue virus (DENV) infection. However, T lymphocytes have also been shown to be important in a protective immune state. In order to investigate the contribution of both humoral and cellular immune responses in DENV immunity, we used an experimental model in which a non-lethal DENV2 strain (ACS46) is used to intracranially prime Balb/C mice which develop protective immunity against a lethal DENV2 strain (JHA1). Primed mice generated envelope-specific antibodies and CD8+ T cell responses targeting mainly non-structural proteins. Immune sera from protected mice did not confer passive protection to na�¯ve mice challenged with the JHA1 strain. In contrast, depletion of CD4+ and CD8+ T lymphocytes significantly reduced survival of ACS46-primed mice challenged with the JHA1 strain. Collectively, results presented in this study show that a cellular immune response targeting non-structural proteins are a promising way in vaccine development against dengue.