Journal of Food: Microbiology, Safety & Hygiene
Open Access

Antimicrobial activity of Araucaria angustifolia seed (pinhao) coat extract and its synergism with thermal treatment to inactivate Listeria monocytogenes

Joint Event on 9^th European Food Safety & Standards Conference & 3^rd International Conference on Food Microbiology & Nutrition

November 29-30, 2018 | Dublin, Ireland

Elaine Biondo, Geferson Henrique Trojaike, Voltaire Sant Anna and Adriano Brandelli

State University of Rio Grande do Sul, Brazil

Posters & Accepted Abstracts: J Food Microbiol Saf Hyg

Abstract :

The objective of the present work is to evaluate the antimicrobial activity of aqueous extract from solid waste of Araucaria angustifolia seed (pinhao) and its synergism with heat treatment against Listeria monocytogenes. A broad spectrum of bacteria and fungi were confronted with pinhão coat extract at concentration of 10 g/L and for the synergism test L. monocytogenes was exposed to temperatures of 55ºC, 60ºC or 70ºC for up to 10 min in the presence of the extract water. Results showed that pinhão coat extract inhibited Staphylococcus aureus, Bacillus cereus, L. monocytogenes, L. innocua and Aeromonas hydrophila growth, but did not inhibit the fungi evaluated. Kinetic modeling showed that L. monocytogenes inactivation followed the first order model. Results showed that the inactivation rate constant increased 105% when L. monocytogenes was treated at 55ºC in the presence of pinhão coat extract. Meanwhile, there was an increase of 58% and 42% at 60ºC and 70ºC, respectively, indicating clear a synergism of the heat treatment with the aqueous extract obtained from the food residue. Thus, pinhão coat extract present antibacterial activity against important foodborne bacteria and its combination with heat processing implies in reduction of the thermal stability of L. monocytogenes, indicating that the combination of both techniques can be an interesting tool to be used in food preservation. Recent Publications: 1. Santâ??Anna V, Biondo E, Kolchinski EM, Silva LFS, Corrêa APF, Bach E, Brandelli A (2017) Total polyphenols, antioxidant, antimicrobial and allelopathic activies of spend coffee ground extract. Waste and Biomass Valorization 8:439-442. 2. Klein B, Biondo, E, Kolchinski EM, Santâ??Anna V (2017) Evaluation of aqueous extracts from agro-industrial residues of pecan nut and pinhão as allelochemical agents. Revista Eletrônica Científica da UERGS 3:495-500. 3. Caxambú S, Kolchisnki EM, Biondo E, Padilha RL, Brandelli A, Santâ??Anna V (2016) Evaluation of the antimicrobial activity of pecan nut [Carya illinoinensis (Wangenh) C. Koch] shell aqueous extract on minimally processed lettuce leaves. Food Science and Technology (Campinas) 36:42-45. 4. Evaluation of the effect of chlorine dioxide on strawberries to control spoilage micro-organisms. Latin American Applied Research. Accepted for publication in 2017. 5. Polesi RG, Rolim R, Zanetti C, Santâ??Anna V, Biondo E (2017) Agrobiodiviversidade e Segurança Alimentar no Vale doTaquari, RS: plantas alimentícias não convencionais e frutas nativas. Revista Científica Rural 19:118-135.

Biography :

Elaine Biondo is graduated in biology from Federal University of Santa Maria (RS, Brazil) and completed his PhD in Botany from Federal University of Rio Grande do Sul. She has published more than 30 papers dealing with on biodiversity and non-conventional edible plants. in reputed journals. Currently, also she has worked at State University of Rio Grande do Sul food microbiology for food and agrochemical applications.

E-mail: elaine-biondo@uergs.edu.br