Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Hyun Ju Kwon, You Na Oh, Soojung Jin, and Byung Woo Kim
Dong-Eui University, South Korea
Posters & Accepted Abstracts: J Proteomics Bioinform
Julbernardia globiflora, a tropical African tree widespread in Miombo woodland, has been used in folk medicine for the treatment of depression and stomach problems. However, the bioactivities of J. globiflora have not yet been fully determined. The objective of this study was to evaluate the antioxidative and anticancer effects of methanol extract of J. globiflora (MEJG) and the molecular mechanism of its anticancer activity in human colon carcinoma HT29 cells. MEJG exhibited significant antioxidative activity and cell growth inhibitory effect on HT29 cells in a dose-dependent manner. MEJG induced apoptosis of HT29 cells with the increase of apoptotic cells and apoptotic bodies using Annexin V staining and 4,6-diamidino-2phenylindole (DAPI) staining, respectively. The MEJG-induced apoptosis was associated with the increase of Fas, a death receptor, and Bax, a pro-apoptotic protein, and the decrease of Bcl-2, an anti-apoptotic protein, resulting in the release of cytochrome c from the mitochondria into the cytosol and activation of caspase-3, -8 and -9. The apoptotic effects of MEJG were confirmed by cleavage of poly (ADP-ribose) polymerase (PARP). Collectively, these results suggest that MEJG may exert the anticancer effect in HT29 cells by inducing apoptosis via both the intrinsic and extrinsic pathways. Recent Publications: 1. Adam J M and Cory S (2007) The Bcl-2 apoptotic switch in cancer development and therapy. Oncogene. 26: 13241337. 2. Fulda S and Debatin K M (2006) Extrinsic versus intrinsic apoptosis pathways in anticancer chemotherapy. Oncogene. 25: 4798-4811. 3. Galluzzi L, Lopez-Soto A, Kumar S and Kroemer G (2016) Caspases connect cell-death signaling to organismal homeostasis. Immunity. 44: 221-231. 4. Li P, Nijhawan D and Wang X (2004) Mitochondrial activation of apoptosis. Cell. 116: 57-59. 5. Riedl S J and Shi Y (2004) Molecular mechanisms of caspase regulation during apoptosis. Nat. Rev. Mol. Cell Biol. 5: 897-907.
Hyun Ju Kwon has completed her PhD in Bioengineering from Osaka University, Osaka, Japan. She is presently working as a Professor at Division of Applied Bioengineering, Biopharmaceutical Engineering Major, Dong-Eui University and as an Assistant Director of Blue-Bio Industry Regional Innovation Center, Dong-Eui University, Busan, Korea. Her research field is Natural Products and Oncology.