Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Mitesh Dwivedi, Digna N Patel, Viral N Pathak, Naresh C Laddha, Mala Singh, Rasheedunnisa Begum and Bankim Desai
Uka Tarsadia University, India
DiaGenic Research Lab Pvt. Ltd., India
In vitro Speciality Lab Pvt. Ltd., India
The Maharaja Sayajirao University of Baroda, India
Rheumatology Clinic, India
Posters & Accepted Abstracts: J Clin Cell Immunol
Rheumatoid Arthritis (RA) is a chronic inflammatory disease of predominantly synovial joints. Angiotensin-converting enzyme (ACE) has been suggested to play a role in pathogenesis of RA, since high levels of ACE have been documented in synovial fluid and pleural effusions. The present study was aimed to investigate association of ACE I/D polymorphism with RA susceptibility, to evaluate the plasma ACE levels in patients and healthy controls and to establish the genotype-phenotype correlation of ACE I/D polymorphism. Polymerase chain reaction (PCR) method was used for genotyping of ACE I/D polymorphism in 103 RA patients and 151 healthy age-matched controls from South Gujarat population. Plasma ACE levels in 34 RA patients and 42 healthy controls were estimated by ELISA. Our results showed that ACE I/D polymorphism was significantly associated with RA. The genotype and allele frequencies for the polymorphism were significantly differed between RA patients and control population (p=0.0017; p=0.0003, respectively). In particular, the susceptible D allele was prevalent in RA group as compared to the control group (71.00% vs. 56.00%). Though, there was no significant difference in the levels of ACE between patient and control groups (p=0.827), the genotype-phenotype analysis for the polymorphism revealed that individuals with DD and ID genotypes exhibit increased ACE levels as compared to the II genotype (p=0.0174; p=0.0062, respectively). The results suggest that ACE I/D polymorphism is associated with RA susceptibility in South Gujarat population and the genotypephenotype analysis indicates that the susceptible D allele may be involved in the pathogenesis of RA. mitesh_dwivedi@yahoo.com