Journal of Clinical Toxicology
Open Access

Case report of a very serious drinking water incident putting up to 50,000 inhabitants of a small town near Bonn in North Rhine- Westphalia, Germany at risk. A concentrated solution of highly alkaline water by sodium hydroxide was accidentally washed into the town’s drinking water at a pumping

4^th Global Summit on Toxicology

August 24-26, 2015 Philadelphia, USA

Marek Murias

Posters-Accepted Abstracts: J Clin Toxicol

Abstract :

Resveratrol and its higher hydroxylated analogues (HHRA) have been reported to possess a variety of biological properties
including antioxidant as well as pro-oxidant activity. The antioxidant properties are assumed to enable these compounds to
protect cells from oxidative damage, however pro-oxidant activity are held likely to be responsible for their cytotoxic or pro-apoptotic
effects. In our studies the effects of resveratrol and HHRA were investigated in various cancer cell lines including breast and cervix
cancer derived cells as well as T cell leukemia Jurkat cells. In our experiments several markers of oxidative stress and apoptosis-linked
events were evaluated. Taken together, resuts of our experiments suggetsed that HHRA possesing ortho-hydroxy groups are stronger
cytotoxic agents than compounds without such a structure. Although these compounds were unstable in our experimental systems,
they exert strong cytotoxic effect, which may be connected with induction of oxidative stress in cancer cells. The effects of oxidative
stress could be shown in our experiments e.g. by accelerated oxygen consumption, increased MDA level or decerased level of GSH.
These events suggest formation of short-living, prooxidative, highly cytotoxic metabolites in cells incubated with HHRA possesing
ortho-hydroxy groups. These effects were less intense when HHRA without ortho-hydroxy groups were used. It was suggested
therefore that HHRA may be grouped, into molecules that can either form quinoid systems upon two electron oxidation (QFS) or
are unable to form such a structure (NQFS). These compounds may be classified as quinone forming QFS, may be used as anticancer
agents while, NQFS represented in this study, presented e.g. by resveratrol may be used mainly as antioxidants.