Journal of Glycobiology
Open Access

Chemical assembly of N-glycans by use of modular building blocks

Glycobiology World Congress

August 10-12, 2015 Philadelphia, USA

Yunpeng Liu

Posters-Accepted Abstracts: J Glycobiol

Abstract :

N-glycans play important roles in biological events such as cell adhesion, cell differentiation, pathogen infection and
immune response. Due to the micro-heterogeneity and low abundance of N-glycans on glycoproteins, there are only
few N-glycans can be isolated from natural resources. As a result, synthesis particularly chemo-enzymatic synthesis has
become an indispensable tool to get access to these compounds. We developed a highly efficient strategy based on the use
of oligosaccharylthioether for the convergent installation of branched GlcNAc-terminated antennae to achieve high stereo
selectivity with excellent yields. This new approach minimizes synthetic steps and maximizes yield which proceeded very
efficiently with less glycosyl donor (1.3 equivalents) and mild conditions (at 0o C). This strategy would allow us to prepare a
series of N-glycans with various glycoforms for enzymatic extension.