Journal of Chromatography & Separation Techniques
Open Access

Chiral separation of Ketoprofen on an achiral NH2 column by HPLC using Vancomycin as chiral mobile phase additive

5^th International Conference on Current Trends in Mass Spectrometry and Chromatography

September 25-26, 2017 Atlanta, USA

Dehbiya Gherdaoui, Hafsa Bekdouche and Michel Righezza

Ecole Normale Superieur de Kouba, Algeria
Aix-Marseille University, France

Posters & Accepted Abstracts: J Chromatogr Sep Tech

Abstract :

A high-performance liquid chromatographic method for chiral separation of Ketoprofen racemate was developed. (R)- and (S)Ketoprofen enantiomers were separated on a LiChrosorb NH2 column (250��4.6 mm, i.d 5 �µm) at 20 �°C, using 2-propanol/ potassium dihydrogen phosphate buffer (pH 6.0, 0.05 M) (50:50 v/v). Containing vancomycin as the mobile phase at a ï¬�ow rate of 0.8 ml min-1 and detection wavelength of UV, the detector was set at 310 nm. Under these conditions, Ketoprofen enantiomers could be separated with a selectivity factor (�±) of 2.172 and a resolution (Rs) of 4.78 using extremely low concentrations of the vancomycin chiral additive.