Medicinal & Aromatic Plants
Open Access
ISSN: 2167-0412
+44 1300 500008
ISSN: 2167-0412
+44 1300 500008
Yamon Pitakpawasutthi, Chanida Palanuvej and Nijsiri Ruangrungsi
Chulalongkorn University, Thailand
Rangsit University, Thailand
Posters & Accepted Abstracts: Med Aromat Plants
Chromolaena odorata (L) R M King & H Rob (syn. Eupatorium odoratum L), known in common name as Siam weed, Christmas bush or common floss flower is a species in family Asteraceae. It has been widely used in traditional medicine for the treatment of skin diseases, diabetes, as well as insect repellent. The leaves of C. odorata from 10 various sources throughout Thailand were investigated for the chemical constituents of essential oil, the content of chlorogenic acid and in vitro antioxidant capacities of this crude drug. The chemical compositions of essential oils were hydro-distilled from the leaves and were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). Chlorogenic acid content was quantitatively analyzed by Thin-Layer Chromatography (TLC) densitometry with winCATS software and TLC image analysis with ImageJ software. The mobile phase for TLC development consisted of ethyl acetate:water:formic acid (17:3:2). Antioxidant activities were investigated by 2, 2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging and �?²-carotene bleaching assays. For the results, C. odorata essential oil showed the major components of pregeijerene, dauca-5, 8-diene, (E)-caryophyllene, �?²-pinene and �?±-pinene. The chlorogenic acid content of C. odorata leaves determined by TLC-densitometry and TLC image analysis were found to be 7.39�?±0.40 and 7.31�?±0.77 g/100 g of dry leaves, respectively. Statistically, the chlorogenic acid content obtained from both methods was not significantly different. DPPH radical scavenging and �?²-carotene bleaching assays of ethanolic extract of C. odorata leaves showed its antioxidant potential.
Yamon Pitakpawasutthi has completed her Bachelor of Thai Traditional Medicine from Prince of Songkla University. She is currently pursuing her PhD from the College of Public Health Sciences, Chulalongkorn University.