Journal of Clinical Toxicology
Open Access

Cochlear cell death induced via cisplatin or gentamycin in combination with furosemide in rodents

2^nd International Summit on Toxicology

October 07-09, 2013 Hampton Inn Tropicana, Las Vegas, NV, USA

Li Xia

Scientific Tracks Abstracts: J Clinic Toxicol

Abstract :

O totoxic models of animal represent an elemental tool in basic otological research. In the present study, using guinea pigs, we compared cochlear lesions mediated via cisplatin applied in terms of two regimens: consecutive application alone and in combination with furosemide. The influences of furosemide alone were also assessed; it was observed to result in temporary hearing loss and reversible damage to the stria vascularis. Consecutive administration of cisplatin alone tended to be disadvantageous because it bred progressive body weight loss and higher mortality compared with the combined regimen, which utilized a smaller cisplatin dose. The combined regimen brought about remarkable hair cell loss without corresponding lesion of spiral ganglion neurons (SGNs). This difference suggests that the co-administered regimen did not mimic the damage to cochlear neuronal innervation caused by clinical application of cisplatin. In the meantime, we spread this method to the ototoxic model build in mice. Co-administration of gentamicin and furosemide caused marked hair cell loss as well as less mortality compared with consecutive application of gentamicin in mice. Due to different pharmacokinetics between cisplatin in guinea pigs and gentamicin in mice, the injection regimens of co-administration of furosemide-cisplatin in guinea pigs and gentamicin? furosemide in mice were varied. Overall, the methods of co-administration of cisplatin/gentacimin and furosemide in rodents facilitate ototoxic model build

Biography :

Li Xia has completed his Ph.D. at the age of 31 from Shanghai Jiao Tong University. Now, he is the staff in the Dept. of Otorhinolaryngology of Shanghai Sixth People?s Hospital. He has published 4 papers in reputed journals. Besides, he has the experience of resident for three years and the experience of working overseas for one year (Ear Lab, Dalhousie University, Canada). He could communicate fluently with the people from English-speaking countries