Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Georgiana Gabriela Iordache, Angela Casarica and Ana Despina Ionescu
National Chemical-Pharmaceutical for Research and Development Institute, Romania
Posters & Accepted Abstracts: J Proteomics Bioinform
Levans are fructose polymers synthesized by a broad range of microorganisms and a limited number of plant species as nonstructural storage carbohydrates and they have potential applications in the pharmaceutical, food, and cosmetic industries (1-5). This study presents a comparative analysis of the growth and consumption curves of Z. mobilis NCIB 1163 and Z. mobilis ATCC 10988, levan producing microorganisms. The growth and consumption curves were performed in a liquid medium with a concentration of 5% sucrose and 5% glucose. Thus, the bacterial cells in the exponential growth phase were centrifuged at 12.000 g and the pellet was washed twice with a sterile 0.9% NaCl solution. Finally, the cells were resuspended in 1 ml of physiological saline and they were used as inoculum in 5% liquid medium, which was monitored concerning the fermentable carbohydrate consumption and the growth. The consumption curves were performed under anaerobic conditions (the culture medium was coated with a layer of sterile paraffin oil) by periodical weighing of the medium seeded with different bacterial strains of Z. mobilis and by the graphical representation of weight loss (due to release of carbon dioxide). Exponentially growing cells were used as the inoculum, made at approximately 107 cells/ml. Cell growth was assayed turbidimetrically at a wavelength of 600 nm. The consumption curves of Z. mobilis NCIB 11163 and Z. mobilis ATCC 10988 bacterial strains on complete sucrose substrate medium, 5% under anaerobic conditions (Figure 1) revealed that strains NCIB 11163 and ATCC 10988 exhibit a similarly kinetics of consumption's substrate. The consumption curves of Z. mobilis NCIB 11163 and Z. mobilis ATCC 10988 bacterial strains on complete glucose substrate medium, 5% under anaerobic conditions (Figure 2) show that glycolysis is more intense than hydrolysis of sucrose. From the curves profile it is observed that the strain ATCC 10988 shows a curve having a more pronounced linear decrease. Recent Publications: 1. Park H-E, (2003) Enzymatic synthesis of fructosyl oligosaccharides by levansucrase from Microbacterium laevaniformans ATCC 15953. Enzyme Microb. Tech. 32: 820-827. 2. Rairakhwada D, (2007) Dietary microbial levan enhances cellular non-specific immunity and survival of common carp (Cyprinus carpio) juveniles. Fish Shellfish Immun. 22: 477-486. 3. Gupta S, (2008) Microbial levan in the diet of Labeo rohita Hamilton juveniles: Effect on nonspecific immunity and histopathological changes after challenge with Aeromonas hydrophila. J. Fish Dis. 3: 649-657. 4. Kang SA, (2009) Levan: Applications and perspectives. pp. 145-161. In: Microbial Production of Biopolymers and Polymer Precursors. Rehm BHA (ed). Caister Academic Press, Norfolk, UK. 5. Jathore, N. R., (2012) Microbial levan from Pseudomonas fluorescens: Characterization and medium optimization for enhanced production. Food Science and Biotechnology, 21(4), 1045��?1053. doi:10.1007/s10068-012-0136-8.
Georgiana Gabriela Iordache has her expertise as a Project Responsible of different Romanian Research Projects related to Heparin Sodium for pharmaceutical and industrial purposes. This work and experience in biotechnology was attained by approximately 10 biotechnological processes, 2 patents and over 3 scientific papers published in specialized journals. She is a PhD student in the field of Biotechnology. She is working as a junior researcher at the National ChemicalPharmaceutical for Research and Development Institute, Bucharest, Romania.