Journal of Clinical Trials
Open Access
ISSN: 2167-0870
ISSN: 2167-0870
Basel Albassett1, Maria Baryshnikova3, Victor Krasnov3 and Ivan Krasnyuk2
Posters-Accepted Abstracts: J Clin Trials
Objective: To compare the cytotoxic effect of anticancer drugs OR-2011 on lyophilized liposomal formulation (LLF) and substance
on human melanoma cells Mel KorandMel Ibr.A new lyophilized liposomal formulation (LLF) OR-2011 was developed for
specialized and profound studies in I.M. Sechenov First Moscow State Medical University and in N.N. Blokhin Russian Cancer
Research Center.
Materials & Methods: A comparison of cytotoxicity of liposomal drugs were performed using the MTT assay based on the
restoration of dehydrogenases of metabolically active of living cells yellow salt 3-[4,5-dimethylthiazol-2]-2,5-diphenyltetrazolium
bromide (MTT) to form a purple crystals of formazan. The amount of formazan obtained depends of the number of cells and their
viability and were determined by photometrically. Cytotoxic effect characterizes the IC50-(concentration of substances that are
caused by the death of 50% of the cells)liposomal formulationOR- 2011 and substances OR-2011 were studied in concentrations
of 1 mg/ml; 0.5 mg/ml; 0.25 mg/ml; 0.125 mg/ml; 0.062 mg/ml; 0.031 mg/mL and 0.015 mg/ml. Cell lines were cultured in a
RPMI-1640 medium containing 10% fetal calf serum (FCS), 10 mM HEPES (Sigma, USA), 2 mML-glutamine (Sigma, USA), 40
ng/ml gentamicin (ICN, USA) 0.1% 1000-fold solution of amino acid and 0.1% 1000-fold rastvorara vitamins (PanE-Co., Russia)
at 37Ã?Â?C in an atmosphere of 5% CO2 and 0.1% 1000-fold vitamin solution (PanEco, Russia), at 37Ã?Â?C in an atmosphere of 5% CO2.
The cells were maintained in logarithmic growth phase of constant reseeding human culture within 3-4 days. For cell detachment
with plastic was used versen solution. Cells were washed with serum-free pure RPMI-1640 medium and transplanted in a 96-well
flat-bottomed plates (Costar, USA) at 4×103 cells 180 μl of complete medium RPMI-1640 per well. To evaluate the cytotoxic effect
of liposomal drugs was added into each well of 20 microliters of various concentrations of drug and incubated with the cells for 24;
48 and 72 hours at 5 %-CO2 incubator at 37Ã?Â?C.
Results & Conclusions: Cytotoxic effect of OR-2011 was most expressed especially in the liposomal forms; after incubating the
cells with the drug for 24 hours, the IC50 for liposomal form has been 0.025 mg/ml and for OR-2011 substances-0.125 mg/ml.
Further at incubation, 48 and 72 hours IC50 for liposomal formulation remained same values and for the OR-2011 substance has
reached 0.062 mg/ml. Thus, study of lyophilized liposomal formulation LLF OR-2011 cytotoxic activity showed more effectively
kills neoplastic cells in comparison with the substance OR-2011.